Efeito do ácido linoleico e catalese sobre o desenvolvimento e criotolerãncia de embriões bovinos produzidos in vitro na ausência de soro e baixa tensão de oxigênio: Mônica Ferreira Accorsi. -

• Ano de defesa: 2014
• Autor(a) principal: Accorsi, Mônica Ferreira [UNESP]
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: Bovino; Acidos graxos; Antioxidantes; Criopreservação; Embriologia veterinaria; Feto - Desenvolvimento; Linoleic acid
• Link de acesso: http://hdl.handle.net/11449/124534; http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/19-06-2015/000836344.pdf

The most commonly recommended way to obtain more resistant in vitro embryos during the cryopreservation process is to change these embryos, whether through removal of fetal bovine serum (FBS), addition of different supplements to the culture medium or changes in oxygen tension in the production process. The objective of this study was to assess the effects of supplementation of the culture medium with linoleic acid and/or catalase in medium without the addition of FBS, in low tension oxygen atmosphere on development, quality and cryotolerance of bovine embryos. In one group, the medium was supplemented with 100 μm of linoleic acid (LA); the second was supplemented with 100UI of catalase (CAT), a third group was supplemented with both, CAT+LA and a fourth group received no supplementation (control), during the entire culture period in an atmosphere of 5% CO2, 7% O2 and N2 88% in BAGS. Cleavage rates did not differ (P>0.05) between groups (72.7; 74.1; 72.1; and 72.0%, respectively). However, embryo production rates in D7 and D8 were different (P<0.05), where the control had the highest blastocyst rate of the treatments and the CAT+LA group presented the lowest rate of production (30.7; 22.4; 19.8; 12.4%). Expanded blastocysts were only foundon D8, indicating a delay in development. There was a significant reduction (P<0.05) of total lipids in the 3 treated groups when compared to the control group. There was no difference in the intracellular levels of ROS between the 4 groups (P>0.05).Total cell number was significantly lower (P<0.05) in the CAT+ LA and the CAT group showed the highest percentage of apoptotic cells. Considering the re-expansion rates in 24 hours (P<0.05), the control group (50%) and the CAT group (67.2%) presented lower rates than the LA group (71.7%) and CAT+LA group (76.7%). At 48 hours, the control group showed the worst rate (35.7%) followed by the CAT group (47.5%)...(Complete abstract eletronic access below)