Variabilidade química infraespecífica e dinâmica de metabólitos secundários fenólicos e diterpênicos em Casearia sylvestris Sw. (Salicaceae): correlação metabolômica e proteômica utilizando cromatografia, espectrometria de massas, ressonância magnética nuclear e quimiometria

• Ano de defesa: 2015
• Autor(a) principal: Bueno, Paula Carolina Pires [UNESP]
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: Produtos naturais; Metabolômica; Proteômica; Cromatografia líquida de alta eficiência; Metabólitos; Natural products
• Link de acesso: http://hdl.handle.net/11449/135955

The species Casearia sylvestris Sw (Salicaceae) is an important representative of the Casearia Jacq. genus, due to its chemical, pharmacological, economic, environmental and biotechnological aspects. Because of its high adaptive capacity, this species is widespread in Central and South America and, in Brazil, it occurs in practically all biomes. The morpho-anatomical, chemical and genetic data available on C. sylvestris, indicate close relationship between the variety lingua and the Cerrado biome, and between the variety sylvestris and Atlantic Forest. Also, there are data describing significant genetic differences between these two varieties. In ecotones Cerrado/Atlantic Forest, it can be found these two varieties, including individuals with intermediate characteristics, considering both morpho-anatomical and genetic ones. There is evidence that in C. sylvestris var. lingua/Cerrado occur predominantly the phenolic compounds, while in C. sylvestris var. sylvestris/Atlantic Forest the clerodane diterpenes predominate. So, the main objective of this work is to evaluate if the chemical composition, considering the secondary metabolites, is related to and/or conditioned by biomes, and mainly associated to their predominant morphotypes and, therefore, under strong genetic control. To evaluate this, an analytical method using UHPLC-DAD was developed and validated for the simultaneous analysis of phenolic compounds and clerodane-type diterpenes produced by the two C. sylvestris varieties. The metabolomic study allowed the identification of 15 clerodane-type diterpenes in C. sylvestris var. sylvestris and 14 glycosylated flavonoids and one catechin in C. sylvestris var. lingua. The study of the circadian rhythm and seasonal cycle of both varieties showed a decrease in the production of the two secondary metabolites classes in the reproductive period. In the infraspecific chemical...