Infecção por Cryptosporidium em bezerros e humanos de assentamentos rurais na região de Araçatuba, São Paulo

• Ano de defesa: 2014
• Autor(a) principal: Matos, Lucas Vinicius Shigaki de [UNESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: Ruminante; Bovino - Doenças; Criptosporidiose; Pessoas; Assentamentos rurais; Persons
• Link de acesso: http://hdl.handle.net/11449/122071

The objectives were to determine the occurrence, evaluate the spatial distribution and molecularly characterize infection by Cryptosporidium spp. in calves and humans living in two rural settlements in the northwestern state of São Paulo, as well as associate positiveness for the parasite to the studied variables. In this study, 231 cattle aged up to six months and 54 people from the two settlements who maintains a close relationship with the animals were examined. Diagnostic positivity of infection by Cryptosporidium spp. was observed in 17 calves by malachite green negative stain and polymerase chain reaction (nested PCR), and by only the latter technique, infection in one person. Statistical analysis of multiple correspondence showed an association between the presence of Cryptosporidium spp. by nested PCR, and watery stools. Analysis of the sequences revealed in 15 samples a similarity to Cryptosporidium andersoni, in one sample a resemblance with Cryptosporidium bovis, and in one sample 100% of identity with Cryptosporidium parvum, being this species detected in the person considered infected. From the results obtained in this study, it can be inferred that, as spatial distribution, in one of the settlements, there was a higher occurrence of Cryptosporidium infection in calves, and in this location a positive human for this parasite was detected. Additionally, in animals up to two months was isolated C. parvum and above this age range, C. bovis and C. andersoni. Complementarily, the aqueous consistency of stools was associated with seropositivity to this parasitic gender