Estudo químico e avaliação da atividade biológica de Eugenia brasiliensis e Eugenia involucrata (Myrtaceae)

• Ano de defesa: 2014
• Autor(a) principal: Dametto, Alessandra Cristina [UNESP]
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: Quimica organica; Flavonoides; Antocianina; Antioxidantes; Agentes anti-inflamatorios; Anthocyanin
• Link de acesso: http://hdl.handle.net/11449/110857

This work deals with the chemical study and evaluation of biological activities of extracts from leaves and fruits of Eugenia brasiliensis LAM. and Eugenia involucrata DC, two Brazilian species used as folk medicines for treating inflammatory diseases. Nineteen anthocyanins were identified from fruits of E. brasiliensis and E. involucrata by HPLC-PDA-MS/MS: cyanidin-3-O-sophoroside (A1), delphinidin-3-O-laminaribioside (A2), delphinidin-3-O-galactoside (A3), delphinidin-3-O-glucoside (A4), cyanidin-3-O-galactoside (A5), cyanidin-3-O-laminaribioside (A6), cyanidin-3-O-sambibioside (A7), cyanidin-3-O-caffeoyl-diglucoside (A8), cyanidin-3-O-glucoside (A9), cyanidin-3-O-caffeoyl-glucoside (A10), delphinidin-3-O-coumaroyl-glucoside (A11), pelargonidin-3-O-glucoside (A12), cyanidin-3-O-maloyl-glucoside (A13), cyanidin-3-O-acetoyl-glucoside (A14), cyanidin-3-O-pentoside (A15), and four non-identified anthocyanins (A16, A17, A18 e A19) and delphinidin (A20) . Except for A3, A4, A5 and A9, which had been previously identified in E. brasiliensis, the other anthocyanis are reported here for the first time in both species. The major anthocyanins, A4 e A9, were quantified and presented yields of 1.15 and 3.01 mg.g-1 to E. brasiliensis (fresh fruit), respectively and 0.58 and 2.02 mg.g-1 to E. involucrata (fresh fruit), respectively. The isolated compounds from E. brasiliensis leaves include one dissacharide (1), two flavanones: 2(S)-7,5-dihydroxy-6-methylflavanone (2) and 2(S)-7,5-dihydroxy-6,8-dimethylflavanone (3), one dihydrochalcone: 2’,6’-dihydroxy-3’-methyl-4’-methoxy-dihydrochalcone (4) and the lignan: 7,8,7’,8’-tetrahydro-8,8’-dimethyl-3,3’-dimethoxy-4,4’-dihydroxidibenzocyclooctadiene (5). Compounds 2, 3 and 4 are reported herein for first time in this species and compound 5 is a new neolignan. Eleven compounds were identified by FIA-MS from the tea of E. brasiliensis and E. involucrata: gallic acid (C1), caffeic acid (C2)...