Influência da suplementação sobre estresse oxidativo e processo inflamatório renal de animais com sobrecarga nutricional

• Ano de defesa: 2014
• Autor(a) principal: Pierine, Damiana Tortolero [UNESP]
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: Rins - Inflamação; Obesidade; Stress oxidativo; Suplementos dieteticos; Kidneys Inflammation
• Link de acesso: http://hdl.handle.net/11449/123252; http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/06-04-2015/000816201.pdf

The mechanisms linking obesity to kidney damage are unknown. The kidney is a target organ lesions caused by AGEs. The receptor of AGEs (RAGE) has proinflammatory stimuli and appears to play a role in the pathogenesis of renal disease. The study aimed to verify the effect of obesity on renal damage and the effect of lycopene on these complications. Male Wistar rats were randomly assigned to receive control diet (C, n = 7) or high fat plus sucrose (HD+S, n = 14) for 6 weeks. After this period, the HD+S animals were randomized into two groups: HD+S (n = 7) and HD+S supplemented with lycopene (HD+S+L, n = 7). The animals received corn oil (C and HD+S+L) and lycopene (HD+S+L, 10 mg/kg/5 times per week) for 6 weeks. At the end of the period 24h urine was collected, the animals euthanized and samples of blood and kidney were obtained for analysis. Biochemical, hemodynamic parameters, renal function, markers of oxidative stress and inflammation were assessed. The adiposity index increased in groups HD+S and HD+S+L (C: 5.8±1.8 < HD+S: 9.4±1.6 = HD+S+L: 9.7±1.7%, p <0.001). The HD+S and HD+S+L animals had insulin resistance (OGTT glucose levels after 150 min, C: 117.6±3.9 < HD+S: 138.1±5.1 = HD+S+L: 137.8±5.2 mg / dL, p = 0.01), but no changes in fasting glucose, lipids, SBP and renal function. Renal concentrations of RAGE and TNF-α increased in the HD+S group and lycopene supplementation restored these increased levels similar to the control group (RAGE values - C: 3.1±0.3 = HD+S+L: 3.1±0.3 < DH+S: 3.6±0.4 mg/g , p = 0.014; TNF-α - C: 227.8±2.7 = HD+S+L: 227.4±2 , 2 < HD+S: 238.7±3.0 ρg/mL, p = 0.014). Lycopene was able to decrease TNF-α RAGE and kidney. Thus, this carotenoid can be beneficial for the prevention and therapy of oxidative stress and inflammation in the kidneys resulting from obesity