Estudo fitoquímico de extratos polares e infusão das folhas de Terminalia catappa L. (Combretaceae) e avaliação de suas atividades antiulcerogênica e mutagênica

• Ano de defesa: 2015
• Autor(a) principal: Mininel, Francisco José [UNESP]
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: Taninos; Espectrometria de massa; Extratos vegetais; Dicroismo circular; Terminalia; Mass spectrometry
• Link de acesso: http://hdl.handle.net/11449/126577

Terminalia catappa Linn. belongs to Combretaceae family and is commonly called almond-the-beach or sun hat.The Combretaceae family gathers numerous species that has been studied for its use by the population for medicinal purposes. It is from Malaysia, but often cultivated and very characteristic of the Brazilian coast. From the alcoholic extract of the leaves were detected as main constituents the major substance punicalagin (α and β anomers), the punicalin compound, gallagic acid and ellagic acid. It was identified as well, the 1,2,3-Tri-O-galloyl-β-D-glucose substances, and possible isomers, 1,6-Di-O-galloyl-β-D-glucose, HHDP acetilglicoside, HHDPhesoside, ellagic acid hexoside, gallagic acid and apigenin 8-C (2 '-galloyl)-β-Dglucopyranosyl. These metabolites were confirmed by FIA-ESI-IT-MSn experiments (Direct Flow Analysis Electrospray ionization-Ion-Trap Tandem Mass Spectrometry) and High Performance Liquid Chromatography (HPLC) coupled with diode array (PDA). Ellagic acid, gallic acid and methyl gallate were confirmed by standard coinjection experiments. Punicalagin was detected and isolated from the hydromethanol fraction as a mixture of anomers experiments and confirmed by 1H and 13C NMR, mono- and bi-dimensional. The ethyl acetate fraction from fractionation of the hydroalcoholic extract by MPLC (Medium Pressure Liquid Chromatography) allowed the isolation of galágico acid metabolites and 8 apigenin-C (2'- galloyl) -β-Dglucopyranosyl. The absolute configuration of anomers of the compound of punicalagin and apigenin 8-C (2'-galloyl) -β-D- glucopyranosyl was established by experiments circular dichroism (CD). Quantitative determination of the main secondary metabolites was performed by HPLC-PDA, thus allowing the determination of concentration and content (%) of metabolites present in the alcoholic extract. They were first quantified in hydroalcoholic extract of this species, the...