Transferência de Salmonella Enteritidis por contaminação cruzada e formação de biofilme em diferentes superfícies de corte

• Ano de defesa: 2014
• Autor(a) principal: Dantas, Stéfani Thais Alves [UNESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: Equipamentos e acessórios - Contaminação; Salmonella enteritidis; Biofilme; Frango de corte; Microorganismos - Desenvolvimento; Equipment and supplies
• Link de acesso: http://hdl.handle.net/11449/110355

The cross-contamination is one of the main factors which contribute to foodborne outbreak. It is estimated that about 60% of foodborne diseases are related to the inappropriate practices, such as cross-contamination involving cutting surface, where the raw chicken is manipulated concurrently to other food. Among the microorganisms responsible by causing foodborne diseases, we can highlight the Salmonella spp., being the serovar Salmonella Enteritidis the one most involved in cases of infection in humans besides being recognized by its capacity to adhere, colonize and biofilm formation on surfaces of contact with food. The biofilm allows the bacteria to develop and grow adhered to surfaces offering resistance to the hygienic process, being able to cause contamination during the food prepare. Thus, 10 strains of S. Enteritidis were used to test the cross-contamination among three cutting surfaces (plastic, wood and glass) and cucumbers before and after hygiene. Besides, these strains were evaluated to their capacity to form biofilm in hydrophobic materials (wood and plastic) and hydrophilic (glass). When the tested surfaces were not sanitized there was the recovering of Salmonella on all the surfaces and in the cucumbers which had contact with these surfaces. Related to the cutting surfaces under sanitized process, the glass was the material with less recovering of the pathogen and the lower occurrence of cross contamination with the cucumbers. Among the 10 strains, three (30%) were not able to produce biofilm in any surface, even in a presence of csgD and adrA genes. The largest percentage of biofilm production occurred on the wood and plastic (60% and 40%, respectively) followed by glass (10%), showing the bacteria adhere better in hydrophobic material. It is possible to conclude that the cutting surfaces represent a critical point in cross-contamination, mainly when they weren’t sanitized. S. Enteritidis was able to form biofilm in ...