Obtenção e avaliação das potencialidades biológicas de complexos metálicos de 'ZN' e 'CO' contendo proteínas hidrolisadas do soro do leite

• Ano de defesa: 2013
• Autor(a) principal: Stefanelli Junior, José Roberto [UNESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: Química inorgânica; Cobalto; Zinco; Soro do leite; Whey
• Link de acesso: http://hdl.handle.net/11449/108482

This study consists of synthesis and evaluation of cobalt and zinc compounds containing proteic hydrolyzates from whey. The motivation of the study is focused on the fact that is a highly nutritive material, which can be used, therefore, as food supply. The recent literature has reported that the bioavailability of trace elements, when administrated in the chelated form, is increased in comparison with the inorganic salts, mostly used as minerals sources. As a secondary objective, it has environmental responsibility to avoid the inappropriate discard of the whey by dairy industries, since it is a highly pollutant product (iological Oxygen Demand of the order of 25,000 - 80,000 mg O2/L). The initial stage of the study was performed in the laboratory of enzymology, Faculdade de Ciências Farmacêuticas of UNESP - Araraquara and consisted in the steps of extraction, treatment, hydrolysis and freezedrying of the whey. The results from SDS-PAGE electrophoresis and aminograms revealed that the enzymatic hydrolysis was effective and led to the release of amino acids. The second stage of the study, performed on Departamento de Química Geral e Inorgânica of this Institute, comprised the synthesis and study of the several compounds obtained containing cobalt or zinc ions and the proteic hydrolyzate, by different synthetic routes and experimental conditions (such as material nature, solvent, pH, temperature, etc.). The major goal of the project is that different coordination compounds containing natural ligands could compose a multimineral list to be tested as metallodrugs (Ag, Pd, Pt) or nutraceutical substances (Cu, Zn, Co, Fe).