Detalhes bibliográficos
Ano de defesa: |
2015 |
Autor(a) principal: |
Sampaio, Laís Simões [UNESP] |
Orientador(a): |
Não Informado pela instituição |
Banca de defesa: |
Não Informado pela instituição |
Tipo de documento: |
Dissertação
|
Tipo de acesso: |
Acesso aberto |
Idioma: |
por |
Instituição de defesa: |
Universidade Estadual Paulista (Unesp)
|
Programa de Pós-Graduação: |
Não Informado pela instituição
|
Departamento: |
Não Informado pela instituição
|
País: |
Não Informado pela instituição
|
Palavras-chave em Português: |
|
Link de acesso: |
http://hdl.handle.net/11449/135958
|
Resumo: |
Brazil has a great botanical diversity, thanks to its various biomes, and the documentation of existing species is very important for understanding the world's biodiversity. Senna genus has 80 species while Casearia genus has 37 species in Brazil. The Lantana genus has about 150 species. Some active compounds of these species were tested in the laboratory presenting promising trypanocidal activity; however there are difficulties in classification of some specimens of these genres. New identification techniques emerge to assist in taxonomy. DNA barcoding is a very useful technique because it is fast, accurate, and highly cost-effective, using a small DNA sequence for identification. Its applications range from identifying cryptic species to the fight against illegal trade in endangered species and illegally harvested timber, among others. To perform this technique, in plants, use genes located in the chloroplast - matK (encoding the protein maturase K) rbcL (encoding ribulose 1,5-bisphosphate or protein Rubisco) and/or intergenic spacer regions in the chloroplast (trbH-psbA). In this work in partnership with NuBBE - Center for Bioassays, Biosynthesis and Ecophysiology of Natural Products, we aimed to identify the species by DNA barcoding technique, using the regions rbcL, trbH-psbA and ITS2, to check the database accuracy and improve the taxonomic classification. The results showed that the rbcL region is a good region for amplification and sequencing, with good species discrimination rate. The psbA-trnH region is less effective in discrimination compared to the rbcL region. The ITS2 region showed identity with fungal sequence, showing difficulty in using this region. |