Criopreservação de oócitos de Prochilodus lineatus (Valenciennes, 1836) (Teleostei, Prochilodontidae)

Detalhes bibliográficos
Ano de defesa: 2015
Autor(a) principal: Ribeiro, Douglas de Castro [UNESP]
Orientador(a): Não Informado pela instituição
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Estadual Paulista (Unesp)
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Link de acesso: http://hdl.handle.net/11449/126410
Resumo: The decades cryobiology seeks to conserve the maternal genome in fish through gamete preservation, but the techniques currently available run into problems such as low permeability of the membranes, the large the amount of yolk and the sensitivity of gametes to cold. Work on the subject has been carried out, however unsystematically and majority form with cold temperate species. Due to lack of information on the subject with Neotropical fish, the aim of this work is to develop a protocol for the freezing of Prochilodus lineatus oocytes for this, was evaluated using different salt solutions (Ringer and Ringer's lactate solution, Hank's balanced salt solutions (HBSS) with and without calcium, and the solution for salmonid eggs) as a means of maintaining and extending the viability of oocytes. We also assessed the toxicity of three cryoprotectants agents (ACPs) the Dimethyl sulfoxide (DMSO), Propylene glycol (PG), and Methanol (MET), to (1) the development of vitrification protocols with exposure, 2.5, 5.0 and 7.5 min. in molarities the 6 M 8 M 10 M CPA; and (2) for the construction of slow cooling protocols with CPAs with increasing incorporation of the baths evaluated toxic effects on fertility in 0.5 M, 1 M, 2 M and 4 M. It was evaluated the lower the sensitivity of oocytes temperature (2.5 ° C) storage on four 30, 60, 90 and 120 minutes. Oocytes underwent slow cooling in nitrogen steam evaluating the morphological injuries due to exposure to the CPAs and freezing. The results showed that only with HBSS without calcium and were able to maintain the state of gametes not activated, however, they do not provide the extent of viability of oocytes, allowing that they continue to suffer from degradation by storage time, similarly to the oocytes retained only in ovarian fluid. The high sensitivity of oocytes to the CPAs and the cold was found. The toxicity of the higher molarities of CPAs (6 M, 8 M and 10 M) at all exposure times, precludes ...