Perfil gênico no oviduto bovino de fêmeas Nelore e Aberdeen angus

• Ano de defesa: 2014
• Autor(a) principal: Fontes, Patricia Kubo [UNESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: Nelore (Zebu); Aberdeen-angus (Bovino); Falópio, Trompas de; Reprodução animal; Expressão gênica; Biotecnologia animal; Fallopian tubes
• Link de acesso: http://hdl.handle.net/11449/113950

The oviduct has an important role in mammal reproduction, promoting a favorable microenvironment for oocyte maturation, sperm storage and capacitation, fertilization, transport of gametes and early embryo development. Anatomically and functionally, the oviduct is divided in three regions: infundibulum, ampulla and isthmus. The oocyte and the sperm enter in opposite sides of the oviduct, respectively infundibulum and isthmus, and are transported to the fertilization site, the ampulla. Reproductive success is directly related to appropriate timing of gamete transport to the fertilization site, as well as a precise time of embryo transport to the uterus, to obtain the capacity of implantation. The coordination and regulation of oviductal functions are complex and under endocrine, paracrine and autocrine effects, which temporally and spatially alter the transcription and translation of several factors. Therefore, this study aimed to evaluate the effect of reproductive biotechnologies, specifically ovarian superstimulation, as well as genetic and physiological reproductive characteristics in the transcriptional profile of several factors in the bovine oviduct. To do so, we evaluated the effects of inducing multiple ovulation in Nelore cows (data presented in the first manuscript), and the effects of the influence of genetic selection of animals with high follicle count in Nellore and Aberdeen Angus heifers, in the initial period post-ovulation (data presented in the second manuscript), in gene expression related to gametes transport and fertilization. The results demonstrated that ovarian superstimulation modulates the expression of some genes related to oviductal contractility in Nelore cows and ovulation is the main factor responsible for transcriptional control in bovine oviduct, with less or no impact of breed and ovarian follicle count