Quantificação de endotoxinas e sua relação com sinais / sintomas e volumetria da lesão periapical e do canal radicular em dentes com infecção endodôntica primária

• Ano de defesa: 2014
• Autor(a) principal: Cardoso, Flávia Goulart da Rosa [UNESP]
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: Endodontia; Endotoxina; Tomografia Computadorizada de Feixe Cônico; Endodontics
• Link de acesso: http://hdl.handle.net/11449/124124; http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/18-05-2015/000828267.pdf

The aims of the present study were: a) quantify endotoxins and bacterial CFU counts in primary endodontic infections before and after biomechanical preparation (BP) and after root canal medication (RCM); b) to correlate the levels of endotoxins and bacterial CFU counts with the volume of periapical bone destruction (VPBD) determined by Cone Beam Computed Tomography (CBCT) analysis as well as with the development of clinical signs and symptoms; c) to correlate the levels of endotoxins and bacterial CFU counts with the volume of the lumen of the canal (VLC); d) to correlate the size of periapical lesions determined by periapical radiographs images with the CBCT images. Thirty teeth with pulp necrosis and apical periodontitis were selected and submitted to CBCT analysis. After access to pulp cavity, a first root canal sampling was performed in order to determine the presence of root canal infection. Afterwards, BP was performed using 2.5% NaOCl followed by the placement of RCM according to the selection group: Calcium Hydroxide [Ca(OH)2] - Ca(OH)2 + Saline Solution (SSL); Ca(OH)2 + Ginger - Ca(OH)2 + 20% glycolic Ginger extract ; Ca(OH)2 + Chlorhexidine (CHX). A second collect were made after the BP and then the third after 14-days of RCM. Culture analysis was performed in order to determine antimicrobial activity. The levels of endotoxins were determined by the Limulus Ameboyte Lysate assay. The VPBD and the VLC were determined by CBCT-analysis and compared to periapical radiographs. Data were typed on a spreadsheet and statistical analyzed. At the baseline samples, CFU counts and endotoxins levels ranged from 0 - 8.16 CFU/mL and 1.75 -149 EU/mL, respectively. Both contents were reduced after BP and after RCM. Positive correlations were found between the levels of endotoxins and VPBD as well as with previous episode of pain. Thereby, a positive correlation was found between the bacterial...