Uso de ultrassom na síntese de diacilglicerol via hidrólise enzimática de óleo de girassol

Detalhes bibliográficos
Ano de defesa: 2015
Autor(a) principal: Raizer, Eduardo lattes
Orientador(a): Cardozo Filho, Lúcio lattes
Banca de defesa: Teleken, Joel Gustavo lattes, Borba, Carlos Eduardo lattes
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Estadual do Oeste do Paraná
Toledo
Programa de Pós-Graduação: Programa de Mestrado em Engenharia Química
Departamento: Centro de Engenharias e Ciências Exatas
País: BR
Palavras-chave em Português:
Palavras-chave em Inglês:
Área do conhecimento CNPq:
Link de acesso: http://tede.unioeste.br:8080/tede/handle/tede/1861
Resumo: Oils and fats are essential foods to the human diet, nevertheless, the negative impact caused by its overconsumption is of great concern worldwide. Diacylglycerol rich oils have orga- noleptic features very similar to those found in conventional edible oils, however, these oils do not tend to accumulate on the body, even when consumed in high quantities, becoming a great resource in the fight against obesity. Sunflower oil is among the most consumed edible oils in the world, and also, is naturally one of the healthiest. This study s goal was to evalu- ate the influence of ultrasound on the enzymatic hydrolysis of sunflower oil with the enzyme phospolipase A1 (Lecitase Ultra) and use mathematical modeling to obtain simulations of production processes of a diacylglycerol rich oil. Ultrasound generates emulsions of water and oil, thus enhancing the system s interfacial area, and producing higher reaction rates compared to conventional enzymatic processes. Sunflower oil partial hydrolysis was carried in a solvent free environment, in 4 hour periods, with temperatures varying from 30 to 50 °C, enzyme/substrate fractions between 0,6 and 4,0% (m/m) and a fixed water/oil fraction of 5% (m/m). with this goal in mind, experimental designs were developed. Results showed optimal reaction conditions on 40 °C and 1,7% (m/m). Subsequently, fixing the optimal parameters, water/oil fraction was evaluated between 2 and 10% (m/m) in a 12 hour period in order to obtain reaction kinetics. Applying a mathematical model to the kinetic curves it was possible to demonstrate that, for the tested fractions, the 2% water/oil ratio is the most favorable to the production of diacylglycerol, and the maximum quantity of this product is obtained at 27 minutes of reaction time.