Uso de ultrassom na síntese de diacilglicerol via hidrólise enzimática de óleo de girassol
Ano de defesa: | 2015 |
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Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Estadual do Oeste do Paraná
Toledo |
Programa de Pós-Graduação: |
Programa de Mestrado em Engenharia Química
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Departamento: |
Centro de Engenharias e Ciências Exatas
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País: |
BR
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Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://tede.unioeste.br:8080/tede/handle/tede/1861 |
Resumo: | Oils and fats are essential foods to the human diet, nevertheless, the negative impact caused by its overconsumption is of great concern worldwide. Diacylglycerol rich oils have orga- noleptic features very similar to those found in conventional edible oils, however, these oils do not tend to accumulate on the body, even when consumed in high quantities, becoming a great resource in the fight against obesity. Sunflower oil is among the most consumed edible oils in the world, and also, is naturally one of the healthiest. This study s goal was to evalu- ate the influence of ultrasound on the enzymatic hydrolysis of sunflower oil with the enzyme phospolipase A1 (Lecitase Ultra) and use mathematical modeling to obtain simulations of production processes of a diacylglycerol rich oil. Ultrasound generates emulsions of water and oil, thus enhancing the system s interfacial area, and producing higher reaction rates compared to conventional enzymatic processes. Sunflower oil partial hydrolysis was carried in a solvent free environment, in 4 hour periods, with temperatures varying from 30 to 50 °C, enzyme/substrate fractions between 0,6 and 4,0% (m/m) and a fixed water/oil fraction of 5% (m/m). with this goal in mind, experimental designs were developed. Results showed optimal reaction conditions on 40 °C and 1,7% (m/m). Subsequently, fixing the optimal parameters, water/oil fraction was evaluated between 2 and 10% (m/m) in a 12 hour period in order to obtain reaction kinetics. Applying a mathematical model to the kinetic curves it was possible to demonstrate that, for the tested fractions, the 2% water/oil ratio is the most favorable to the production of diacylglycerol, and the maximum quantity of this product is obtained at 27 minutes of reaction time. |