Otimização da produção de xilanase de Penicillium crustosum por planejamento experimental e aplicação no biobranqueamento da polpa celulósica

Detalhes bibliográficos
Ano de defesa: 2014
Autor(a) principal: Silva, Nyéssia Fernanda de Sousa lattes
Orientador(a): Kadowaki, Marina Kimiko lattes
Banca de defesa: Simão, Rita de Cássia Garcia lattes, Ernandes, Samara lattes
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Estadual do Oeste do Parana
Programa de Pós-Graduação: Programa de Pós-Graduação em Ciências Farmacêuticas Mestrado
Departamento: Ciências Farmacêuticas
País: BR
Palavras-chave em Português:
Palavras-chave em Inglês:
Área do conhecimento CNPq:
Link de acesso: http://tede.unioeste.br:8080/tede/handle/tede/10
Resumo: The aim of this study was to optimize the production of xylanase by Penicillium crustosum using Plackett-Burman the Design (BDP) and Central Composite Rotational Design (CCRD), and its application in the bleaching process of kraft pulp. The PBD-12 was carried out to screening the significant variables of the compounds of the culture medium: NaNO3; KH2PO4; MgSO4 7H2O; KCl; Fe2(SO4)3, yeast extract, corn stover and initial pH under liquid static culture at 28 °C for 6 days. The variables corn stover, KH2PO4, and pH were significant at p <0.10 by DPB. Statistical analysis of the results obtained with CCRD exhibited the three variables (KH2PO4 0.15%, corn stover 2% and initial pH 6.0) that showed significant effects at p <0.05, and the maximum production of xylanase was 50 U/mL, 14 times higher compared to enzyme activity before optimization. The treatment of the kraft pulp with P. crustosum xylanase showed a significant reduction in kappa number (5.27 Kappa points and efficiency (35.04%). Thus, there is evidence of the potential application of xylanase produced by P. crustosum in the bleaching process of kraft pulp in paper industry