Inibição de Klebsiella pneumoniae carbapenemase por micocinas produzidas por Wickerhamomyces anomalus

• Ano de defesa: 2019
• Autor(a) principal: Nascimento, Bruna Larissa
• Orientador(a): Gandra , Rinaldo Ferreira
• Banca de defesa: Gandra , Rinaldo Ferreira , Sanches , Andréia Cristina Conegero , Peder , Leyde Daiane de
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual do Oeste do Paraná; Cascavel
• Programa de Pós-Graduação: Programa de Pós-Graduação em Ciências Farmacêuticas
• Departamento: Centro de Ciências Médicas e Farmacêuticas
• País: Brasil
• Palavras-chave em Português: Atividade antimicrobiana; Toxina killer; Resistência bacteriana
• Palavras-chave em Inglês: Antimicrobian activity; Killer toxin; Bacterial resistance
• Área do conhecimento CNPq: FARMACIA::FARMACOGNOSIA
• Link de acesso: http://tede.unioeste.br/handle/tede/4388

The killer system consists of killer yeast secretion of mycocins. These substances have the potential to affect other sensitive microorganisms. Wickerhamomyces anomalus is a yeast that produces mycocins target of studies in different areas, including the environment, industry and medical sciences. Klebsiella pneumoniae carbapenemase is highlighted by antimicrobial resistance profile. Due to this problem of multiresistant bacteria, new treatments alternatives have been studied. Among them are the mycocins, these have already demonstrated effectiveness in the inhibition of diverse fungi and bacteria. This work aims to verify the inhibition of Klebsiella pneumoniae carbapenemase by mycocins produced by Wickerhamomyces anomalus. Β-glucanases were determined, microdilution assays in liquid and solid medium and haemolysis test. Wickerhamomyces anomalus supernatant showed β-glucanase activity of 2.36 U/mg. Results showed antimicrobial activity in 100% of strains of Klebsiella pneumoniae carbapenemase up to the concentration of 0.12 U/mg. At the concentration of 0.06 U/mg, 36.7% of the strains were inhibited and at a concentration of 0.03 and 0.02 U/mg 3.3% of the strains were susceptible to mycocins. In addition, the hemolysis test showed low cytotoxicity of the mycocins, with a concentration of 1.18 U/mg 5.4% concentration higher than the concentrations capable of inhibiting 100% of the Klebsiella pneumoniae carbapenemase strains.Thus, we can note the potential of Wickerhamomyces anomalus mycocins in the development of new antimicrobial substances.