Desenvolvimento de método analítico por espectroscopia de fluorescência para determinação de bisfenol A

• Ano de defesa: 2018
• Autor(a) principal: Zabotti, Caroline
• Orientador(a): Bariccatti, Reinaldo Aparecido
• Banca de defesa: Bariccatti, Reinaldo Aparecido , Rosa, Maurício Ferreira da , Colla, Eliane
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual do Oeste do Paraná; Toledo
• Programa de Pós-Graduação: Programa de Pós-Graduação em Química
• Departamento: Centro de Engenharias e Ciências Exatas
• País: Brasil
• Palavras-chave em Português: Complexos de inclusão; β-Ciclodextrina; Espectroscopia de fluorescência
• Palavras-chave em Inglês: Inclusion complexes.; β-Cyclodextrin; Fluorescence spectroscopy
• Área do conhecimento CNPq: QUIMICA ANALITICA::ANALISE DE TRACOS E QUIMICA AMBIENTAL
• Link de acesso: http://tede.unioeste.br/handle/tede/4010

Analytical methodologies for the quantification of bisphenol Metastases are analytical for the quantification of bisphenol. This monitoring if by the consumer this is the environmental pollutant which has been applied in health. In the work in question, we investigated the use of a bisphenol inclusion complex as a strategy for the determination of traffic levels of solutions by spectrofluorimetry. The use of the molecular encapsulation of β-cyclodextrin with the analyte of interest provided a high detection capacity (0.03 ppb) due to an elevation of the inclusion level, which provided higher stiffness for the bisphenol molecule response of fluorescence intensity, without interference of noise or impaired analytical signal. A simple and sensitive spectrofluorimetric method is presented. Linearity was investigated in the range of 3.85 to 26.5 ppm, the source coefficient being among the very significant variables, 0.996. The retention limit was 0.03 ppb for the β-cyclodextrin effect, and the limit of quantification was 7.5 ppm, which was a lower intensity where precision and accuracy were investigated. The method was considered accurate and accurate in the absence of the matrix, since it was verified in the sample selection, there are several unknown results present in the aqueous matrix that provoke internal filter effects. Robustness was evaluated through applications of a central rotational compound design, where the effect of the variables studied in the analytical method was analyzed. The quantification of bisphenol A by fluorescence spectroscopy proved to be a suitable method, easy to learn and fast.