Control perspectives of the Cattle Tick Rhipicephalus microplus and hemoparasites Babesia bovis and Anaplasma marginale

• Ano de defesa: 2017
• Autor(a) principal: Forero Becerra, Elkin Gustavo
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: eng
• Instituição de defesa: Universidade Federal de Viçosa
• Palavras-chave em Português: Carrapato como transmissor de doenças; Rhipicephalus microplus; Bovinos - Doenças; Bovinos - Vacinação; Doenças Parasitárias de Animais
• Link de acesso: http://www.locus.ufv.br/handle/123456789/9913

Recently, R. microplus has extended its distribution to higher altitudes in Colombia. Proper identification of acaricide resistance mechanisms is required, as well as tick- borne diseases diagnosis and vaccine candidates for Babesia bovis and Anaplasma marginale. Patent restrictions and the scholarship funding requirements established three separated, but related, chapters for the DSc thesis. Chapter 1: Detection of genes to synthetic pyrethroids resisntance and Babesia spp. in Rhipicephalus microplus and cattle blood samples from Colombia. Samples of R. microplus (engorged females and larvae) and cattle blood were collected from selected farms in 8 municipalities in Colombia. A PCR screening for mutations on CzEst 9 and IIIS6 genes that confer synthetic pyrethroid resistance showed all tick samples had a heterozygous genotype to CzEst9 and most of the samples had an IIIS6 susceptible genotype. A nested PCR screening for detection of B. bovis rra and B. bigemina rap-1c genes resulted in unexpected bands prevented conclusive evidence. Chapter 2: Transformation process of Pichia pastoris KM71 with the synthetic gene H1Bbo23290 derivative from Babesia bovis RAP-1 protein. The transformation process of Pichia pastoris KM71 with H1Bbo23290 (a promising vaccine candidate against bovine babesiosis) was unsuccessful. Plasmid DNA extraction and subsequent linearization were the major obstacles. Chapter 3: Bovine immune response produced by oMP7, oPM8, and oMP9 Outer Membrane proteins from Anaplasma marginale St. Maries strain in confined experimental immunized animals. OMP7, OMP8, and OMP9 proteins were used to test potential conserved sequences containing CD4 T-cell epitopes. Using in vitro T-cell proliferation assays to test recombinant A. marginale and A. centrale OMP7, OMP8, and OMP9, and their overlapping peptides spanning each protein, conserved immunogenic T-cell epitopes were been identified in some peptides.