Physiological and metabolic characterization of adenylates carriers in Arabidopsis thaliana

• Ano de defesa: 2021
• Autor(a) principal: Silva, Roberto Neri da
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: eng
• Instituição de defesa: Universidade Federal de Viçosa
• Palavras-chave em Português: Arabidopsis thaliana - Efeito do stress; Adenilato Quinase; Bioinformática; Repetições palindrômicas curtas agrupadas e regularmente espaçadas; Fisiologia Vegetal
• Link de acesso: https://locus.ufv.br//handle/123456789/28244

Adenylate transporters play a vital role in animal and plant cells, connecting ATP production sites to consumption regions. So far, 16 of these transporters have been identified in plants. Although progress has been made in recent years regarding the understanding of the functions performed by these proteins, there are still gaps to be filled. In this context, this work initially presents a study of the role of these proteins in plant responses to a series of different types of stress. Here, using bioinformatics data, we demonstrate that the expression pattern of adenylate transporters is variable in different environmental conditions, suggesting that these genes have distinct and non- redundant functions in plants. In the second chapter of the thesis, we investigated the function of mitochondrial adenylate transporter ADNT1 in A. thaliana plants under flooding conditions. ADNT1 deficiency caused greater leaf loss and a lower maximum quantum yield of PSII electron transport. Stress responses occurred prematurely in ADNT1 deficient plants, due to increased levels of alanine and other stress marker metabolites, such as aspartate, trehalose, and inositol. We also observed increased expression of SnRK1, ADK, APY, PDC1, and PPDK genes, indicating that the disturbance in the adenylate pool caused by ADNT1 deficiency caused alterations in gene expression and metabolism related to stress responses. In the third chapter, The CRISPR / Cas9 system was used through the vector pKAMA-ITACHI (pKI1.1R) to obtain mutant plants to characterize the APC1 transporter in A. thaliana. We observed that APC1 mutant plants have a shorter silique length and a smaller number of seeds. Furthermore, APC1 deficiency led to the production of short roots and a reduction in seedling fresh weight in a medium without sucrose. Together, our results suggest that the APC1 transporter plays an important role in reproductive organs, and seedling development. Keywords: ATP. Stress responses. Bioinformatic. CRISPR/Cas9.