Effects of B vitamins and hydroxy trace minerals supplementation on hepatic metabolism of beef cattle at finishing phase

• Ano de defesa: 2021
• Autor(a) principal: Lopes, Mariana Mescouto
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: eng
• Instituição de defesa: Universidade Federal de Viçosa
• Palavras-chave em Português: Bovinos de corte; Complexo de vitamina B; Minerais na nutrição animal; Metabolismo energético; Proteômica; Avaliação de Alimentos para Animais
• Link de acesso: https://locus.ufv.br//handle/123456789/27968

Vitamin B and trace minerals are crucial molecular signals involved in many biological pathways of energy metabolism, immune response, and others, being their bioavailability compromised in high-producing ruminant animals. Despite the current knowledge of the effects of vitamin B complex and trace minerals on animal performance, their use as a rumen-protected form and its impact on liver metabolism in finishing beef cattle is poorly known. The present study aimed to assess the effects of rumen-protected B-vitamin blend and hydroxy trace minerals on the hepatic proteome. A total of 20 non-castrated Nellore males with 353 ± 43 kg of initial body weight were randomly assigned to one of the following treatments: CTRL – inorganic trace minerals without supplementation of protected vitamin B blend; SUP – supplementation of hydroxy trace minerals (Cu and Zn) and protected vitamin B blend (B5, B6, B7, B9, and B12). All animals were fed whit the same level of the experimental diet for 106 days and liver biopsy was performed at the end of the experimental period. We use shotgun proteomics combined with biological and network analyses of the protein differentially abundant between treatments, showing 37 proteins differentially abundant (P < 0.10) between treatment groups, where all proteins were up-regulated in the SUP treatment. These proteins were related to protein folding (P = 0.04), mitochondrial respiratory chain complex I (P = 0.01), and IV (P = 0.01), chaperonin-containing T-complex 2 (P = 0.01), glutathione metabolism (P < 0.01) and others linked to oxidative stress response. These results indicate that rumen-protect vitamin B and hydroxyl trace minerals supplementation during the finishing phase alters the abundance of proteins associated with the electron transport chain and other oxidation- reduction pathways, boosting the production of reactive oxygen species. Such alteration appears to modulate proteins linked to oxidative damage response to maintain cellular homeostasis. Keywords: Beef cattle. Proteomics. Vitamin B. Hydroxy trace minerals. Liver metabolism