Effects of dietary nucleotides supplementation on physiological and growth performance responses of LPS - challenged broiler chickens

• Ano de defesa: 2018
• Autor(a) principal: Kreuz, Bruna Strieder
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: eng
• Instituição de defesa: Universidade Federal de Viçosa
• Palavras-chave em Português: Frango de corte - Alimentação e rações; Imunoglobulinas; Lipopolissacarídeos; Nucleotídeos; Nutrição e Alimentação Animal
• Link de acesso: https://locus.ufv.br//handle/123456789/27143

Nucleotides play crucial roles in the storage and transfer of genetic information, cell division and protein synthesis. The aim of this study was to evaluate the effects of dietary nucleotides supplementation on growth performance, body temperature, serum biochemical parameters, immunoglobulin G (IgG) levels, immune organ weight, and intestinal morphometry of broiler chickens challenged with Escherichia coli lipopolysaccharide (LPS). Ninety-six 7-day-old broiler chicks (Cobb 500) were randomized in a β х β factorial design composed of two dietary types (supplemented with 0 and 0.3 g of free nucleotides/kg of feed) and two inflammatory conditions (without and with LPS administration), with 8 replicates per treatment and 3 birds per cage. The experimental period was divided into two phases: 7-20 days (initial; before LPS challenge), and 21-35 days of age (final). The LPS challenge consisted of repeated intraperitoneal injection of LPS at 21, 23, 25, and 27 days of age. The initial dose of 1 mg/kg of body weight was increased by 12% at each subsequent injection to circumvent endotoxin tolerance. Data were analyzed using a one-way and two-way ANOVA for initial and final phases, respectively. During the initial phase, nucleotides supplementation resulted in greater (P < 0.05) body weight and average daily gain (ADG), and better (P < 0.05) feed conversion ratio. In addition, birds supplemented with nucleotides had greater (P < 0.05) serum IgG concentrations. During the final phase, LPS challenge resulted in decreased (P < 0.05) ADG and average daily feed intake, and in greater (P < 0.05) body temperature. Nucleotides supplementation increased (P < 0.05) serum IgG concentration on day 28 and LPS challenge increased (P < 0.05) serum IgG concentration on day 28 and 35. At 35 days of age, broilers supplemented with nucleotides had greater (P < 0.05) villus height than their control counterparts. In summary, dietary nucleotides supplementation improves growth performance and serum IgG level in healthy young broilers, and improves gut integrity in 35-day-old broilers.