Avaliação in vitro e in vivo do efeito da cobertura de biossilicato aplicado sobre a superfície de titânio durante a osseointegração

• Ano de defesa: 2013
• Autor(a) principal: Oliveira, Maiolino Thomaz Fonseca
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Uberlândia; BR; Programa de Pós-graduação em Odontologia; Ciências da Saúde; UFU
• Palavras-chave em Português: Osseointegração; Implantes dentários; Biovidro; Implantes dentários osseointegrados; Osseointegration; Dental implants; Bioglass; CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA
• Link de acesso: https://repositorio.ufu.br/handle/123456789/16968; https://doi.org/10.14393/ufu.di.2013.103

The aim of this study was to evaluate in vivo, using beagle dog model, the effect of incorporation of biossilicato (highly bioactive glass) on the process of osteogenesis through the bone-implant contact and evaluate in vitro using model cell culture, the influence of the incorporation of biossilicato the rough surface on titanium discs adhesion and cell viability. The implants used for the in vivo study (n = 32) were 10 mm long and 3.5 mm in diameter and having surface treated with double etching. Half of these implants (n = 16) received the incorporation of biossilicato on its surface roughened (Group AA + BIO), while the other half only with rough surfaces (n = 16) was used as control group (group AA). The in vitro assays used commercially pure titanium disks grade IV 8.0 mm diameter by 4.0 mm in height with the same characteristics of implants used in in vivo tests. The assessment from scanning electron microscopy revealed that about 25% of the implant surface was covered by highly bioactive glass so homogeneous. Surface double etching associated biossilicato (AA + BIO) also showed a lower contact angle (α10s = 5.3 ± 1.7 °) compared with the AA surface. The adhesion test used to verify the stability of highly bioactive glass particles embedded on both disks as implants, demonstrate adequate stability, with no disruption of the interface bond between the particle and the surface of titanium. The results of in vitro through cell culture, showed that the percentage of viable cells adhered to the roughened surface covered with biossilicato (AA + BIO) was significantly lower (p <0.001) than the group with surface - AA (P = 0.004). The cell viability test showed that there were no statistically significant differences between groups AA and AA + BIO in any of the periods analyzed. The histomorphological results suggest that the initial repair is not affected by surface AA + BIO compared with the surface only with double etching.