Efeitos de concentrações crescentes de glicerol sobre a atividade da n-acetil glicosaminidase

• Ano de defesa: 2008
• Autor(a) principal: Garrote Filho, Mario da Silva
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Uberlândia; BR; Programa de Pós-graduação em Genética e Bioquímica; Ciências Biológicas; UFU
• Palavras-chave em Português: N-acetil-β-D-glicosaminidase; Trichoderma harzianum; Glicerol; Uréia; Enzimas; glycerol; urea; CNPQ::CIENCIAS BIOLOGICAS::GENETICA
• Link de acesso: https://repositorio.ufu.br/handle/123456789/15798

Experiments performed with the enzyme N-acetyl-β-D-glucosaminidase (NAGase), secreted by the fungus Trichoderma harzianum, reveal that glycerol concentrations of 0,5 and 1,0 mol.L-1 increase the enzyme activity until a maximum value. Between 1,5 and 2,0 mol.L-1, glycerol decreases the enzyme activity. This effect was observed at different temperatures of the interval between 30 and 70 °C and also in the presence of increasing urea concentrations until 2,0 mol.L-1. The decrease in enzyme activity was larger in the presence of urea and glycerol than solely in the presence of urea. A model based in the concepts of microstates and ensembles was elaborated to explain the glycerol behavior.