Desenvolvimento de plataforma biofotônica para detecção plasmática da sepse: Estudo pré-clínico de prova de conceito

• Ano de defesa: 2023
• Autor(a) principal: Vilarinho, Glauciane Silva
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Uberlândia; Brasil; Programa de Pós-graduação em Ciências da Saúde
• Palavras-chave em Português: ATR-FTIR; biomarcadores plasmáticos; plasmatic biomarkers; sepse, triagem; sepsis, screening; diagnóstico; diagnosis; CNPQ::CIENCIAS DA SAUDE; Ciências médicas; ODS::ODS 12. Consumo e produção responsáveis - Assegurar padrões de produção e de consumo sustentáveis.
• Link de acesso: https://repositorio.ufu.br/handle/123456789/39964; http://doi.org/10.14393/ufu.di.2023.528

Sepsis is a systemic response with different clinical stages against an infectious agent such as bacteria, viruses, fungi, or protozoa. Currently, early diagnosis presents a clinical challenge to reduce its high mortality rate, in addition to generating a high cost of treatment in public and private health sectors. In this context, the present study search to identify potential plasmatic biomarkers based on a biophotonic technology by ATR-FTIR aiming at screening or diagnosing sepsis in a preclinical study. For this, a murine model of sepsis was established by cecal ligation and puncture (CLP). Sixteen rats were randomly divided into control (n=8) and sepsis (n=8) groups. The animals were euthanized 24h after sepsis induction and blood was collected. Blood plasma was analyzed using an ATR-FTIR platform and univariate and multivariate analyzes were used to screen for potential spectral biomarkers of sepsis in plasma. The presence of sepsis was confirmed by an increase (p < 0.05) in C-reactive protein concentration compared to controls. In the absorbance normalized spectrum, the band area between 2945-2888 cm-1 was reduced (p<0.05) in animals with sepsis compared to controls. The analysis of the band area in the region 2945-2888 cm-1 indicated a sensitivity of 87.5% and a specificity of 75%. In the second derivative spectrum, the vibrational mode at 1513 cm-1 was also reduced (p<0.05) in animals with sepsis compared to controls. Analysis of the height of the valley at 1513 cm-1 indicated a sensitivity of 75% and specificity of 87.5%. In conclusion, these results indicate that ATR-FTIR spectroscopy supported with univariate analysis has the potential to provide an alternative for the detection of sepsis without the use of reagents, sustainably, and rapidly using blood plasma samples.