Avaliação e caracterização de matriz extracelular colagenosa descelularizada como implante subcutâneo em modelo murino

Detalhes bibliográficos
Ano de defesa: 2020
Autor(a) principal: Barbosa, Matheus Carvalho
Orientador(a): Não Informado pela instituição
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de Uberlândia
Brasil
Programa de Pós-graduação em Imunologia e Parasitologia Aplicadas
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Link de acesso: https://repositorio.ufu.br/handle/123456789/31137
http://doi.org/10.14393/ufu.di.2020.126
Resumo: With the growing demand to develop and evaluate materials for regenerative medicine and tissue engineering, the present study aimed to verify the applicability of a new porcine decellularized scleral tissue as a subcutaneous implant. The sclera is an essential connective ocular tissue, which plays a key role in supporting the eye structure, as well as protecting nearby ocular tissues. Its main composition is of water, collagen, elastin, proteoglicans, glycoproteins and fibroblasts. The biomaterial was characterized by its structure and main composition, along with a decellularization protocol in order to remove cellular content and allow xenogeneic implantation. DNA extraction and histology proved the efficacy of the decellularization process. Scanning electron microscopy and hydroxyproline assay were used to assess the morphological structure and main composition of the tissue, indicating great collagen content. Furthermore, decellularized extracellular matrix sections were implanted in rats to evaluate the inflammatory response and behavior of the implant. Results showed presence of inflammation, playing a key role in cicatrization as well as formation of a new tissue, along with the development of fibroblastic and angionenic activities, suggesting tissue remodeling and integration. This way, the decellularized extracellular matrix may have potential to be considered as a biomaterial for tissue engineering and regenerative medicine fields.