Caracterização das atividades biológicas da proteína recombinante P21 de Trypanosoma cruzi sobre linhagem celular tumoral de mama in vitro

• Ano de defesa: 2015
• Autor(a) principal: Borges, Bruna Cristina
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Uberlândia; BR; Programa de Pós-graduação em Biologia Celular e Estrutural Aplicadas; Ciências Biomédicas; UFU
• Palavras-chave em Português: Trypanosoma cruzi; RP21; Anti-tumoral; CNPQ::CIENCIAS BIOLOGICAS::MORFOLOGIA::CITOLOGIA E BIOLOGIA CELULAR
• Link de acesso: https://repositorio.ufu.br/handle/123456789/12403; https://doi.org/10.14393/ufu.di.2015.15

Several studies involving Trypanosoma cruzi infection have shown anti-tumor characteristics of the parasite and some of its proteins. A protein with a molecular weight of 21kDa was sequenced, cloned and characterized as being a protein secreted by the parasite which is directly involved in cell invasion by amastigote and trypomastigote forms, this protein was named P21. Studies carried out with recombinant form of this protein, rP21 shows that it has some biological activities which may some beneficial role of this protein in inflammatory processes and even the development of tumors. Breast cancer is the second most common cancer in the world and the experimental model used most commonly to represent is the Ehrlich tumor. This study aimed to evaluate the effects of recombinant protein rP21 of T. cruzi in Ehrlich tumor cells. Initially, the cell viability was analyzed using Ehrlich cells and peritoneal macrophages from BALB/c mice exposed to different concentrations of rP21 in different exposure times and both types of cells remained viable at all times and the protein concentrations. The effect of this chemoattractant protein was evaluated by an invasion assay, which demonstrated that the protein was not able to induce cell invasion Ehrlich, however, the protein was able to induce the invasion of peritoneal macrophages. After that, the capacity of the polymerization of actin cytoskeleton by rP21 was analyzed, showing that the protein is capable of polymerization cytoskeleton in a dose-dependent manner. It was also seen that the solid Ehrlich tumor model, treatment with rP21 shown to inhibit tumor growth. From these features, the biological activities of the protein rP21 are restated, showing the possibility of use of the protein in the anti-tumor treatment.