Produção de alérgenos recombinantes relacionados a Der p 1 de Dermatophagoides pteronyssinus com potencial aplicação na avaliação da resposta imune humoral e celular em pacientes com alergias respiratórias

• Ano de defesa: 2011
• Autor(a) principal: Tomaz, Laura Fontes
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Uberlândia; BR; Programa de Pós-graduação em Ciências da Saúde; Ciências da Saúde; UFU
• Palavras-chave em Português: Alergia; Dermatophagoides pteronyssinus; Alérgenos recombinantes; Imunologia; Allergy; Recombinant allergens; CNPQ::CIENCIAS DA SAUDE
• Link de acesso: https://repositorio.ufu.br/handle/123456789/12714

Dermatophagoides pteronyssinus (Dp) is an important specie of house dust mite associated with allergic diseases. Allergens from Dp are important causes of sensitization and mediated responses to specific immunoglobulin E (IgE) among patients with asthma and allergic rhinitis. The aim of this study was to produce recombinant protein Der p 1 and Der p 1-related IgE binding epitopes (rDer p 1172-243) to evaluate their reactivity to IgE in the serum of mite sensitized individuals using enzyme immunoassays. The sequences of rDer p 1 and rDer p 1172-243 were amplified and subcloned into E. coli. After expression and purification the reactivity of IgE to rDer p 1 and rDer p1172-243 was determined by ELISA. The results showed that rDer p 1 expressed in E. coli presented an estimated molecular weight of about 26 kDa and rDer p 1172-243 about 8kDa. The expressed rDer p 1 protein and rDer p 1172-243 reacted positively with IgE in 88% and 84% of sera from allergic patients, respectively. However, sera from negative patients showed crossreactivity to rDer p 1, although the same was not observed for rDer p 1172-243. It can be suggested that rDer p 1172-243 are promising candidates for the diagnosis of allergy caused by D. pteronyssinus in mite sensitized patients.