Estudo experimental, comparativo, controlado, randomizado e mascarado entre uso de mitomicina C e injeção subconjuntival de bevacizumab, conjunta e isoladamente na cirurgia antiglaucomatosa em coelhos

• Ano de defesa: 2018
• Autor(a) principal: Hilgert, Christiana Velloso Rebello [UNIFESP]
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo (UNIFESP)
• Palavras-chave em Português: Bevacizumab; Iop; Glaucoma/surgery; Filtering surgery; Wound healing; Rabbits; Mitomicina; Cirurgia filtrante; Cicatrização
• Link de acesso: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=6747679; https://repositorio.unifesp.br/handle/11600/52868

Objective: To determine the effects of bevacizumab combined or not with Mitomycin C (MMC) on intraocular pressure and scarring process in experimental glaucoma filtration surgery in rabbits. Methods: This is a maskedobserver experimental study. Thirty New Zealand female rabbits underwent modified glaucoma filtration surgery and were allocated into three groups to receive intraoperatively in Group A:subconjunctival bevacizumab, Group B: MMC and subconjunctival bevacizumab and in Group C: MMC. Intraocular pressure was measured on immediate preoperative period and on postoperative days 8, 14, 17, 21, 26 and 30. Scarring process was addressed 30 days after the surgery by tissue section using Hematoxylin Eosin, Masson’s Trichrome and Picrosirius. Vascular Endothelial Growth Factor (VEGF) expression was addressed by immunohistochemical analyses. Results: Group A had higher intraocular pressure compared with B and C (P<0.01) from the 8th to 13th postoperative day. No significant differences between groups B and C were found. The amount of fibrosis were similar with all stains used in groups A, B and C. There was less VEGF expression on Group A comparing to groups B and C (p<0.01). There was no difference between group B and C regarding VEGF expression. Conclusion: Bevacizumab associated with MMC had lower IOP means, but it was not statistically significant when compared to MMC alone. The amount of fibrosis were similar among groups. A higher inhibition of VEGF was found when bevacizumab was used alone, rather than when combined with MMC.