Prospecção dos efeitos biológicos do Poligodial isolado da Drimys brasiliensis (Winteraceae) na via de sinalização da insulina e no estresse oxidativo em células hepáticas

• Ano de defesa: 2015
• Autor(a) principal: Gomes, Moisés Felipe Pereira [UNIFESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo
• Palavras-chave em Português: JNK; Reactive oxygen species; HepG2; 2-deoxy-d-ribose; Diabetes; Espécies reativas de oxigênio; Deoxi-d-ribose
• Link de acesso: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=3276264; https://repositorio.unifesp.br/handle/11600/47112

According Brazilian Diabetes Society (SBD, 2006), "Diabetes mellitus" is not a single disease but a heterogeneous group of metabolic disorders has in common hyperglycemia, which is the result of defects in insulin action in secretion or both. Sustained hyperglycemia promotes extensive deleterious effects and sometimes irreversible to the body as renal failure, retinopathy, cardiovascular and liver diseases, thereby impairing the regulation of metabolism of carbohydrates. Due to this insulin resistance in the early stages of type 2 diabetes mellitus (DM2), the sensitive lipase hormone (LHS) does not have its activity suppressed by this hormone, thus promoting increase in free fatty acids (FFA) Current that will be drained into the hepatic portal system, thus promoting non-alcoholic fatty liver disease (NAFLD). In traditional medicine, Drimys brasiliensis infusions are used for the treatment of diabetes. However, there are no reports on the polygodial (PGD), the major compound of D. brasiliensis, could be responsible for the hypoglycemic effect. Therefore, we analyzed cell viability by hepatocytes (HepG2) grown with PGD (30, 300 and 3000nM) and evaluate the antioxidant activity in cultured cells with 2-deoxy-D-ribose (2-drib) (50mM) for aindução the oxidative stress. We also study the effect on the insulin receptor, Akt and JNK, as well as their phosphorylated forms. Our results demonstrate that: 1) the PGD does not have antioxidant activity; 2) Treatment with PGD presents a trend in decreased expression of AKT and 3) has a synergistic effect with PGD 2-drib, leading to an increase in the phosphorylation of JNK. We conclude that diabetics who use tea D. brasiliensis may have worsening of symptoms due to the deleterious effect of PGD in enhanced oxidative stress.