Efeito do tratamento com extrato padronizado de Ginkgo biloba (EGb) sobre a microbiota intestinal, o proteoma e o metabolismo do tecido adiposo branco de ratos com obesidade induzida pela dieta

• Ano de defesa: 2018
• Autor(a) principal: Hirata, Bruna Kelly Sousa [UNIFESP]
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo
• Palavras-chave em Português: Adipose tissue; Phytotherapic; High-fat diet; Microbiota; Tecido adiposo; Fitoterápico; Dietas hiperlipídicas; Extrato padronizado de Ginkgo biloba
• Link de acesso: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=7360448; https://repositorio.unifesp.br/handle/11600/52850

Over the last 30 years, the nutritional transition has allowed the incidence of obesity to reach epidemic levels. In this context, the elucidation of new treatments becomes desirable. Lately, the standardized Ginkgo biloba extract (GbE) has been described as a potential agent in the treatment of obesity and metabolic diseases improving insulin resistance in diet-obese rats. In this way, the present study aimed to describe possible mechanisms of action involved in its beneficial effects previously observed. For this purpose, diet-induced obesity rats were treated with EGb for 14 days and the proteome and metabolism of retroperitoneal and epididymal depots of white adipose tissue were evaluated, together with the evaluation of the composition of gut microbiota. The intake of high-fat diet promoted an increase of body mass even with the reduction of food intake. After treatment, GbE reduced food intake, however, this decline did not reflect a reduction in body weight gain and adiposity. Interestingly, EGb reduced the volume of visceral adipocytes and the rate of acetate and oleate incorporation comparing to obese non-treated rats. In addition, GbE reduced the gene expression of key enzymes involved in lipid metabolism such as perilipin 1 and fatty acid synthase (FAS), as well as it decreased FAS protein levels. Furthermore, EGb modulated the expression of 25 proteins, increasing the expression of citrate synthase and reducing the expression of inflammatory proteins (complement C3, mast cell protease and Ig gamma) and proteins involved with oxidative stress (peroxiredoxin). GbE also stimulated the antioxidant catalase activity and decreased levels of lipid peroxidation. In relation to intestinal microbiota, GbE was able to modulate its composition, supporting the growth of Bacteroidetes in detriment of Firmicutes. In conclusion, the present data demonstrates that GbE plays a beneficial effect on lipid metabolism, adipose tissue proteome and gut microbiota of obese rats, corroborating previous studies from our laboratory in which it was proposed GbE as a promising therapy to treat obesity and related disorders.