Identificação de genes diferencialmente expressos nas vias de neurotransmissão cerebral de camundongas anovulatórias

• Ano de defesa: 2013
• Autor(a) principal: Azevedo Junior, Marcos Antonio [UNIFESP]
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo (UNIFESP)
• Palavras-chave em Português: gene expression; anovulatory; neurotransmission; expressão gênica; anovulatória; neurotransmissão
• Link de acesso: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=889486; https://repositorio.unifesp.br/handle/11600/46869

Women with pre-eclampsia may present thrombocytopenia, a severe sign of the disease. The aim of this study was to evaluate the platelet aggregation capacity in pre-eclampsia women. Platelet function was studied in 20 non-pregnant women, 25 normotensive pregnant women, and 20 pre-eclampsia women. Pre-eclampsia was diagnosed by association of hypertension (? 140 x 90 mmHg) and proteinuria (? 300 mg per 24 hours). Platelet was counted in an hemocytometric chamber, and thrombocytopenia was considered when platelet number was lower than 186x103/?l, that represents the average minus two standard deviation of non-pregnant women values. The platelet aggregation was measured in an aggregometer after ADP and collagen agonists stimulation. The comparison among the groups studied was done by one way analysis of variance followed by the Tukey test and P values than 5% were taken to be significant. A significant difference was seen in platelet count among non-pregnant (284+49x103/?l) and pregnant women (normotensive: 219+58x103/??l versus pre-eclampsia: 211+54x103/??l). The platelet aggregation induced by ADP was significantly higher in non-pregnant women (87.7+6.1) than normotensive pregnant women (73.6+14.9) and pre-eclamptic women (53.8+21.7), while the values from this last group was lower than in the normotensive pregnant grou Platelet aggregation in response to collagen stimulation was similar among non-pregnant (90.4+5.4) and normotensive pregnant women (83.4+11.1) and lower in pre-eclamptic women (67.7+23.4). In pre-eclamptic women the platelet aggregation induced by ADP and collagen was similar in the groups with or without thrombocytopenia. The results suggest that pre-eclamptic women platelets have weaker ability to aggregate independent of the number. Thus, platelet function may be altered even when their number is normal in pre-eclampsia.