Polycomb & Trithorax uma análise da expressão gênica do cérebro de camundongos fêmeas obesos transplantados com tecido adiposo gonadal de fêmeas magros

• Ano de defesa: 2017
• Autor(a) principal: Freitas, Eduardo Henrique da Silva [UNIFESP]
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo (UNIFESP); Brasil
• Palavras-chave em Português: Polycistic Ovarian Sindrome; Obesity; Polycomb and trithorax complexes; Leptin; Fat female mouse xxiii; Drosophila proteins; Disease models, animal; Sindrome do ovário policístico; Obesidade; Complexos polycomb e trithorax; Leptina; Camundongo fêmea obeso; Proteínas de drosophila; Proteínas do grupo polycomb; Modelos animais de doenças
• Link de acesso: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=5766150; http://repositorio.unifesp.br/handle/11600/50949

The Polycystic Ovary Syndrome (PCOS) is the most common androgenic disorder in women during reproductive life. PCOS may also be accompanied by metabolic syndrome and recent studies points to leptin as playing a role in disrupting infertility and in changing the energy balance in obese mice through its action on the hypothalamus. The aim is to assess the expression of the Polycomb & Trithorax Complexes genes in the brain of mice, transplanted with fat tissue from normal mice, to better understand the neuronal molecular mechanisms underlying the reversion of PCOS. Brain material of four B6 V-Lepob/J mouse groups is used: norma! weight, obese (control), 7 days treated obese and 45 days treaded obese. AH have their brain RNA extracted and submitted to an 84 Polycomb & Trithorax complexes genes PCR array plate and MetaCore™ software for canonical pathways localization. Genomic profiles obtained are compared to the ones of the control group and presented differentially expressed genes of 25% and 10,7% at 7 and 45 days, respectively, after the treatment. At 7 days, 90,5% presented hyper-regulated and 9,5% hypo-regulated and, finally, at 45 days 100% presented hyper-regulated. The major changes occur in genes: Snail l 36,1; Smarcal l -14,3; Ctbp2 l 5,7; Ezh1 i 5,65; Rnasel l 5,51 and Dnml3b l 5,50. Expressly-altered genes are associated to canonical pathways and provide three networks related to epigenetics. Underlying neuronal changes caused by íeptin in obese-mice brain, there is an important role played by the histone code. Here is shown evidence that leptin drives the chromatin packing to a more condensed pattern. Upregulation of methyltransferase genes, like Ezh1, favors this thought. In summary, the Polycomb & Trithorax complexes might answer for the silencing of some downregulated genes in the obese mice brain when exposed to leptin.