Filmes de Langmuir e Langmuir-Blodgett de lipídios contendo pectinase

• Ano de defesa: 2021
• Autor(a) principal: Rodrigues, Raul Torres [UNIFESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo (UNIFESP)
• Palavras-chave em Português: Langmuir-Blodgett; Langmuir; Pectinase; Carbon; Nanotubes; Enzima; Nanotubos De Carbono
• Link de acesso: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=10432292; https://repositorio.unifesp.br/handle/11600/68090

In this work, we incorporated the enzyme pectinase into Langmuir monolayers of phospholipid acid dimyristoyl phosphatidic (DMPA) and transferred it to solid supports by the Langmuir-Blodgett (LB) technique. We also combine carbon nanotubes with mixed Langmuir films and investigate their interference with the physicochemical properties of ultrafine films. The enzyme of choice in this work is easily produced from filamentous fungi type A. niger. DMPA was chosen because it is an anionic lipid that can have interesting interactions with the enzyme in the study pH range and carbon nanotubes were used because they are known in the literature as enhancers of the analytical signal and the preservation of the enzymatic structure. The supramolecular structure of the Langmuir monolayer was stabilized by the incorporation of the enzyme from the formation of metastable structures. The presence of carbon nanotubes gave the films an enzyme activity superior to that of the enzyme incorporated into the DMPA monolayers. The results presented here show an alternative in the perspective of the development of new bioinspired films for biosensing applications aimed at analytical purposes.