Estudos bioquímicos em camundongos C57BL/6 submetidos à variação da concentração de treonina na dieta

• Ano de defesa: 2016
• Autor(a) principal: Bruno Neto, Giuseppe [UNIFESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo (UNIFESP)
• Palavras-chave em Português: Methylglyoxal; Threonine; Pathways of methylglyoxal formation; Elimination of methylglyoxal; Standardization of diet; Metilglioxal; Treonina; Vias de formação do metilglioxal; Eliminação do metilglioxal; Padronização de dieta
• Link de acesso: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=3775674; https://repositorio.unifesp.br/handle/11600/47289

Methylglyoxal (MGO) is an alpha-dicarbonyl compound endogenously synthesized by metabolism which has several proposed routes of synthesis in the body, among them, from the essential amino acid threonine. MGO also has cytotoxic effects in the body, and its concentration is regulated through the cycle of the glyoxalases that transform it into lactate. With the interference in some metabolic pathways, some alteration in the alpha-dicarbonyl synthesis can be provoked, being able to modify the concentrations of this compound in the organism. Therefore, the objective of this work is to standardize an experimental model through the provision of a diet for male C57Bl / 6 mice, altering one of the MGO synthesis pathways by interfering with the concentration of threonine available in the diet of the animals. Weighing and evaluation of feed consumption were performed weekly and glycemic index evaluation at the end of the protocol. The method of determination of reduced Glutathione (GSH) and Oxidized Glutathione (GSSG) was validated and later these compounds were quantified in the animals' livers. The profile of the metabolites present in the liver was also evaluated by direct infusion in a mass spectrometer (Q-TOF). The weights of the animals treated with threonine (CT) 24 ± 1.67 g and 30 ± 2.24 g, were higher than those animals submitted to the diet with absence of threonine (ST), 11 ± 1.79 g 21 ± 1.17 g. Regarding the GSH and GSSG levels, there was no significant difference (p <0.05) between the groups. Values ??ranged from 0.83 ± 0.62 mmol / g to 1.44 ± 0.36 ?mol / g GSH for the CT groups for 60, 80 and 90 days respectively, and ranged from 1.43 ± 0.15 mmol / G, at 0.07 ± 0.08 mmol / g GSH for the ST treated groups for the same periods. GSSG concentrations also showed no differences between groups ranging from 0.38 ± 0.20 mmol / g to 0.31 ± 0.11 mmol / g for the CT groups and 0.28 ± 0.12 mmol / g , At 0.61 ± 0.33 mmol / g, for the ST groups. The method of Glutathione determination was validated with satisfactory results, with a LOD and LOQ below 0.1 mmol / ml for GSH and GSSG. The alterations observed by the standardization of the experimental model of the animals showed that the interference in only one of the MGO pathways had an effect on the metabolism of the animals and on the behavior of the metabolites present in the liver.