Interferência da cimetidina na histofisiologia androgênica de epidídimos de ratos

• Ano de defesa: 2017
• Autor(a) principal: Santi, Fabiane de [UNIFESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo (UNIFESP)
• Palavras-chave em Português: Anti-androgen; Apoptosis; Androgen receptor; Karyopherin; Abp; Cimetidine; Androgen-binding protein; Sex hormone-binding globulin; Rats; Cimetidina; Antiandrogênico; Apoptose; Receptor de andrógeno; Shbg; Importina; Carioferinas; Proteína de ligação a androgênios; Globulina de ligação a hormônio sexual; Ratos
• Link de acesso: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=5001201; http://repositorio.unifesp.br/handle/11600/50199

Hormonal disorders and changes in the reproductive tract have been related to antiandrogenic effect of cimetidine. Thus, androgen-dependent structural and functional parameters of epididymis were investigated. Male rats received 100mg/kg cimetidine (CMTG) or saline (CG) for 50 days, and the epididymal cauda region was processed for light and transmission electron microscopy. Epididymal duct diameter and stromal collagen were evaluated. TUNEL-labeled epithelial cells, and AR, KPNA and SHBG immunolocalization were analyzed. In CMTG, the duct diameter reduced and a high number of TUNEL-labeled cells was associated to apoptotic features. In the epithelium, a weak/absent AR nuclear immunofluorescence was observed in contrast to enhanced AR-immunolabeled cytoplasm and reduced KPNA immunoreactivity. The collagen and SHBG levels reduced in the stroma. Cimetidine induced epithelial androgenic dysfunction due to impairment of AR nuclear import. These changes may be result of androgen unavailability in the stroma and/or to a direct cimetidine antiandrogenic effect on epithelial cells.