Biomonitoramento citogenético em usuários de enxaguatórios bucais: análises in vitro e in vivo

• Ano de defesa: 2010
• Autor(a) principal: Carlin, Viviane [UNIFESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo (UNIFESP)
• Palavras-chave em Português: Ensaio em cometa; Mucosa bucal; Testes de mutagenicidade; Testes para micronúcleos
• Link de acesso: http://repositorio.unifesp.br/handle/11600/9146

The aim of the present study was comparatively evaluate DNA damage and cellular death in cells exposed to various commercially mouthrinses available: Listerine®, Cepacol®, Plax alcohol free®, Periogard®, Plax Whitening®. A total of 75 volunteers were included being distributed into five groups containing 15 people each for in vivo study. Exfoliated oral mucosa cells were collected immediately before the mouthrinse exposure and after 2 weeks. Furthermore, blood samples were obtained from 3 healthy donors for in vitro study. Ten microliters of the tested mouthrinse was then added to human peripheral blood cells for 1 hour at 37°C. After that, all treatments were incubated with MMS at 10 μmol/L concentration or H 2 O at 100 μmol/L concentration. The negative control cells were treated with PBS for 1 hour at 37°C. The results showed that Periogard promoted an increase of micronucleus frequency after continous exposure. Plax whitening induced extensive genetic damage in peripheral blood cells after in vitro exposure. Listerine was able to reduce genetic damage induced by hydrogen peroxide. Taken together, the results of the present study suggest that Periogard and Plax Whitening are able to induce genetic damage whereas Listerine is an antioxidant agent.