Estudo da avaliação antiparasitária de extratos e constituintes químicos das folhas de Petiveria alliacea L. (phytolaccaceae)

• Ano de defesa: 2015
• Autor(a) principal: Sessa, Deborah Pelosi [UNIFESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo
• Palavras-chave em Português: Phytolaccaceae; Petiveria alliacea; Phytochemical study; Flavonoids; Triterpenes; Antiparasitic activity; Leishmaniasis; Estudo fitoquímico; Flavonóides; Triterpenos; Atividade antiparasitária; Leishmaniose
• Link de acesso: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=3283769; http://repositorio.unifesp.br/handle/11600/47569

This work reports the phytochemical study of leaves from Petiveria alliacea L. (Phytolaccaceae), aiming the characterization of antiparasitic compound. The hexanic and methanol extracts from the leaves were subjected to successive chromatographic procedures to afford two triterpenes: oleanolic (I) and ursolic (II) acids, three flavonoids: sakuranetin (IV); quercetin (V) and patuletin (VI) as well as one glycosylated: kaempferol-3-O-?-L-rhamnopyranosyl-(1?4)-?-D-glucopyranoside (III), being the first occurrence of III, IV and VI in P. alliacea. The structures of substances were characterized through analysis of 1H and 13C NMR and MS spectra followed by the comparison of reported data. Extracts and purified compounds were evaluated for their antiparasitic potential, against L (L.) infantum (amastigote and promastigote), indicating that compounds I ? V demonstrated activity for amastigotes. Compounds II and III showed IC50 values determined as 13.4 and 17.3 ?g/mL, respectively, and lower than to the positive control pentamidine (22.1 ?g/mL). Additionally compounds I, II, V + VI and V showed reduced toxicity against mammalian cells LLC-MK2 lineage. Therefore this study are intended to provide new prototypes for development of new drug for the treatment of parasite diseases.