Influência da ingestão de juçara (Euterpe Edulis Mart.) associada ou não à dieta hiperlipídica sobre a ativação de áreas do sistema nervoso central envolvidas na homeostase energética

• Ano de defesa: 2018
• Autor(a) principal: Reis, Natalia dos [UNIFESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo (UNIFESP)
• Palavras-chave em Português: Obesidade; Juçara; Homeostase energética; Sistema dopaminérgico; Hipotálamo; Obesity; Energy homeostasis; Dopaminergic system; Hypothalamus
• Link de acesso: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=6855187; https://repositorio.unifesp.br/handle/11600/52322

The search for strategies to combat or treat obesity has great value given the epidemic proportions of this pathology. The fruit of the juçara palm (Euterpe edulis Mart.) is rich in phenolic compounds, with anti-inflammatory and antioxidant properties. In a previous study, we have shown that juçara improves glucose tolerance and insulin sensitivity in obese mice, as well as reduces weight gain, an effect associated with increased energy expenditure. However, the association of juçara to a standard diet increased both food intake and body weight. To better understand the actions of this fruit, our objective is to evaluate the effects of the juçara on the activation of neurons of hypothalamic nuclei and the dopaminergic system, responsible for the control of energy homeostasis. For this purpose, C57bl/6 mice (n=5/group) fed either standard (C) or high-fat (H) diet in combination (CJ and HJ, respectively) or not with juçara (0.5%) for 16 weeks was used. At the end of the experiment, the juçara increased body weight in the control group (CJ> C) and decreased in the high-fat group (HJ <H). The food efficiency was higher in the H and HJ groups in relation to C and CJ, and lower in the HJ group compared to the H group. By the immunoperoxidase method for deltaFosB protein, preliminary results of activation of encephalic areas involved in energetic homeostasis were obtained. High-fat diet increased deltaFos expression in the cingulate cortex (H and HJ > C and CJ) but no effect was observed in the prelimbic and infralimbic cortex. Juçara supplementation increased deltaFos expression in the hypothalamic arcuate nucleus (CJ > C, HJ > H). In the dorsal hypothalamus, deltaFos expression was reduced in the H group compared to the others (C, CJ and HJ). DeltaFos expression was also evaluated in the lateral, medial and paraventricular hypothalamic nucleus but no difference among the groups was observed. In the nucleus accumbens, juçara supplementation increased deltaFos in mice fed control diet (CJ>C). High-fat diet fed mice also had increased deltaFos expression (H>C). However, juçara supplemented in high-fat diet kept deltaFos expression in the HJ group similar to C group (H>HJ). In the ventral tegmental area juçara supplementation increased deltaFos expression (CJ>C and HJ>H). Conclusions: our results show that juçara supplementation, in the dosage we used, increases deltaFos expression in both homeostatic and hedonic areas related to the control of food intake. The effect on the reward system is more pronounced when juçara is associated with control diet.