Função de Dicer no tecido adiposo para performance e efeitos metabólicos do exercício aeróbico em camundongos

• Ano de defesa: 2016
• Autor(a) principal: Brandao, Bruna Brasil [UNIFESP]
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo (UNIFESP)
• Palavras-chave em Português: Exercício aeróbio; Processamento de micrornas; Tecido adiposo; Metabolismo oxidativo; Performance
• Link de acesso: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=3883471; http://repositorio.unifesp.br/handle/11600/48478

MicroRNA processing in adipose tissue declines with aging in mice and humans and this is associated with downregulation of the endoribonuclease Dicer. This phenomenon can be prevented by catabolic stimulus as dietary restriction and plays a role in the onset of age-related metabolic diseases in mice. Here we hypothesize that exercise could also regulate this endoribonuclease. We found that eight-week moderate exercise increases Dicer expression by 2-3-fold in different adipose tissue depots of mice. Additionally, AMPK is necessary for this increase since we did not observe it in fat-specific AMPKa1/2 knockout. Dicer upregulation was required for the metabolic adaptation of exercise since fat-specific Dicer knockout mice (AdicerKO) had exercise training-induced increases in performance and adipose tissue oxidative capacity abrogated. Moreover, trained AdicerKO mice had impaired mitochondrial function in adipose tissue, leading to an increase in anaerobic glycolysis and accumulation of lactate in this tissue. Our data indicate that exercise upregulates Dicer in adipose tissue, in a AMPK-dependent manner, to promote oxidative metabolism and confer adaptive metabolic effects.