Envolvimento das proteínas fosforiladas no resíduo de tirosina no hipocampo de ratos submetidos ao status epilepticus induzido por pilocarpina

• Ano de defesa: 2016
• Autor(a) principal: Castro Neto, Eduardo Ferreira de [UNIFESP]
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo (UNIFESP)
• Palavras-chave em Português: Epileptogenesis; Epileptic status; Phosphotyrosine proteins; Protein microarray; Growth factor; Epileptogênese; Estado de mal epiléptico; Miroarranjo de proteínas; Fatores de crescimento
• Link de acesso: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=3635061; http://repositorio.unifesp.br/handle/11600/46763

Protein phosphorylation on tyrosine residue has been associated with different pathologies in the nervous system, including temporal lobe epilepsy. Objective: The objective of this work was to identify the phosphotyrosine proteins involved in status epilepticus (SE) onset/maintenance and those linked to SE resistance in animals submitted to pilocarpine-induced epilepsy. Method: Three groups of animals were studied: SE Group (rats with 5 h of SE, n-=5); PSS group (pilocarpine-injected animals that did not develop SE, n=5) and Control Group (saline-treated rats, n=5). The hippocampi were isolated and submitted to proarray kit, (Full Moon BioSystems, Inc.) containing specific antibodies to identify 228 phosphoproteins. Data were analyzed using ANOVA and Tukey- Kramer as post-test and p ? 0.05 was accepted. Results: One hundred twenty three out of 228 phosphoproteins presented significant altered levels among groups. From these 123 phosphoproteins, 36 attracted our attention due to their important particularities, that is, 18 presented increased phosphorylation compared to control and non-SE groups, 12 from non-SE group displayed significant differences when compared to control and SE groups and 6 presented altered levels among all groups. Conclusion: In this study we identified proteins that are probably linked to the generation of SE from those linked to the SE resistance. Knowing pathways involved in epileptogenesis may help to understand the mechanisms responsible for different vulnerabilities to long-lasting seizures.