Modelos de estudo de melanoma murino: reprogramação em estado pluripotente induzido e a identificação de células com fenótipo tronco

• Ano de defesa: 2019
• Autor(a) principal: Camara, Diana Aparecida Dias [UNIFESP]
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo (UNIFESP)
• Palavras-chave em Português: Melanoma; Reprogramming; Heterogeneity; Cancer Stem Cell; Pluripotency Factors.; Reprogramação; Heterogeneidade; Células Tronco Tumorais; Fatores De Pluripotência
• Link de acesso: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=8753682; https://repositorio.unifesp.br/handle/11600/59757

Malignant melanoma is a heterogeneous tumor, originating from melanocytes that undergo genetic and epigenetic changes. Many studies indicate the existence of a cellular subpopulation that would be responsible for tumor growth and maintenance. Such cells are tumor stem cells (CSCs), because in addition to giving rise to phenotypically different cells, they still express the characteristic stem cells. In addition, the cells undergo changes and may migrate to disorders of tumor focus, at the same time as they become resistant to chemotherapy. This article, the diagnosis the cells can be of great interest in oncology, which whirling therapies have been directed to these cells. Therefore, this work aims to produce, identify, isolate and characterize cell populations with characteristics of stem cells, in order to evaluate the ability to originate heterogeneous populations and their associations factors related to pluripotency. Key