Efeito do desacoplamento da fosforilação oxidativa na criopreservação do sêmen humano

Detalhes bibliográficos
Ano de defesa: 2018
Autor(a) principal: Lima, Bruna Tuani De [UNIFESP]
Orientador(a): Não Informado pela instituição
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de São Paulo (UNIFESP)
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Link de acesso: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=7256833
https://repositorio.unifesp.br/handle/11600/52858
Resumo: Aim: To evaluate the possible protective effect of the decoupling of oxidative phosphorylation and concomitant stimulation of glycolysis during cryopreservation of human spermatozoa. Methods: Each semen collected through the spermogram was divided into two fractions that received the following treatment: (1) simultaneous treatment of oxidative phosphorylation and stimulation of glycolysis (treated group); The aliquots were cryopreserved and analyzed, before and after this process, through evaluations of oxidative status, structural integrity and sperm motility. The obtained data were evaluated and the level of significance used to reject H0 (null hypothesis) was 5%. Results: The aliquots received from the Treated Group showed more cells with intact acrosomes, less DNA fragmentation and better mitochondrial activity compared to the Control Group. Conclusions: From the results obtained, we can conclude that the treatment had a protective effect on human spermatozoa during cryopreservation: (1) preventing cryoinjury, such as high DNA fragmentation and decreased acrosome integrity; (2) maintaining the integrity and activity of mitochondria.