Efeitos da suplementação de vitamina D sobre a pressão arterial, metabolismo glicídico, responsividade vascular e função renal de ratos espontaneamente hipertensos submetidos à dieta hiperlipídica palatável

• Ano de defesa: 2016
• Autor(a) principal: Geraldini, Simone [UNIFESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo (UNIFESP)
• Palavras-chave em Português: Vitamin D; Palatable hyperlipidic diet; Metabolic syndrome; Spontaneously hypertensive rat; Endothelial dysfunction; Renal function; Vitamina D; Dieta hiperlipídica palatável; Síndrome metabólica; Rato espontaneamente hipertenso; Disfunção endotelial; Função renal
• Link de acesso: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=4813569; http://repositorio.unifesp.br/handle/11600/48059

Background: Vitamin D insufficiency has been associated with several diseases such as obesity, diabetes mellitus and hypertension. However, the cause-effect relationship of vitamin D supplementation on dysfunctions and abnormalities of metabolic syndrome has not been clearly elucidated. Objective: Analyze the effects of cholecalciferol supplementation on blood pressure, glucose metabolism, body weight, renal function and vascular relaxation in spontaneously hypertensive rats (SHR), with palatable hyperlipidic diet (PHD)-fed. Methods: Male 12-weeks-old SHR rats were divided into four groups: control SHR (SHR-C), SHR+PHD (SHR-OB), SHR+vit.D (SHR-VD), SHR+PHD+vit.D (SHR-OBVD). Cholecalciferol supplementation was performed by gavage (1000 UI/kg/daily), and the animals were treated for 12 weeks. Weekly, body weight and tail blood pressure (TAP) were measured. At the end of 12 weeks, Oral Glucose Tolerance Test (GTT) and Insulin Tolerance Test (ITT) were performed. Proteinuria was determined in the baseline and the end period, 25-hidroxivitamina D serum and clearance of creatinine were determined in the end of treatment. The vascular endothelium-dependent function was evaluated in isolated rings of the thoracic aortas, also the relative visceral fat weight was performed. Results: The SHR-OB group presented lower body weight gain compared to the SHR-C and SHR-OBVD groups (p=0,02). However, both treated groups with PHD showed higher epididimal fat than the groups standard rat chow-fed (p<0,001). The vitamin D was not able to reduce final TAP as well as shift maximal responses of endothelium-dependent vascular relaxation. Besides, vitamin D treatment attenuated insulin resistance only on SHR-VD group, in the same way decreased the proteinuria only in this group (p=0,001). Conclusion: An oral supplementation of vitamin D can be an adequate strategy for delay the development of glucose metabolism dysfunctions in metabolic syndrome when associated with a balanced diet, avoiding therefore the loss of renal function.