Análises metabolômica e de peroxidação lipídica do líquido folicular de pacientes submetidas ao tratamento de fertilização in vitro somente com FSH e com adição de LH

• Ano de defesa: 2018
• Autor(a) principal: Costa, Livia Do Vale Teixeira da [UNIFESP]
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo (UNIFESP)
• Palavras-chave em Português: Infertilidade; Metabolômica; Estímulo hormonal; Estresse oxidativo; Espectrometria de massas
• Link de acesso: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=7498369; https://repositorio.unifesp.br/handle/11600/52911

Objective: To assess the effect of exogenous LH addition during ovarian stimulation in the metabolomic profile and lipid peroxidation levels in the follicular fluid of women undergoing IVF. Methods: A retrospective selfparing study was performed in which 36 patients were submitted to assisted reproduction treatments under different ovarian stimulation protocols. The patients were divided into two groups: FSH group, composed of 3 6 follicular fluid samples of patients that received FSH only as gonadotropin in the first treatment; and LH group, composed of 36 samples of the same patients who returned to the assisted reproduction section and received a new stimulus, in which LH was added to treatment. Results: The analysis of the clinical data showed no statistical difference in the clinical parameters of the patients when LH was added. The metabolomic analysis showed that there was a different pattern of metabolites among the same patients submitted to different stimuli, determined by the presence of 5 categories of lipids: fatty acids, sphingolipids, glycerophospholipids, prenols and sterols. Analysis of lipid peroxidation showed that there was no statistical difference between the groups. Conclusion: From the results obtained, we could observe that there is a change in the pattern of metabolites when the exogenous LH is added to the ovarian stimulus. This finding may help in determining the most efficient protocol for each patient and, later, in the determination of therapeutic targets for personalized treatments.