Estudo do disco epifisário de camundongos fêmeas lactantes com hiperprolactinemia e seus filhotes

• Ano de defesa: 2020
• Autor(a) principal: Araujo Junior, Osvaldo Pereira [UNIFESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo (UNIFESP)
• Palavras-chave em Português: Hyaline Cartilage; Bone; Epiphyseal Growth Plate; Offspring; Lactating; Hyperprolactinemia; Cartilagem Hialina; Osso; Placa De Crescimento Epifisário; Camundongo Filhote; Lactação; Hiperprolactinemia
• Link de acesso: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=10239906; https://hdl.handle.net/11600/64820

Objective: To evaluate the epiphyseal plate growing (EPG) of offspring de lactating mice with hiperprolactinemia induce by metoclopramida. Materials and Methods: For this purpose, 20 adult female mice were divided into 2 groups with 10 animals/each: control group (GI, Ctr-L) – received the vehicle (0.2 mL saline) and treated group (GII, HPrl-L) – received metoclopramide (200 µg/day dissolved in vehicle). All animals received subcutaneous injections for 50 consecutive days. After 50 days 10 females of each group were placed for mating with males and continued to receive treatment (during pregnancy until 10th day of breastfeeding). The offsprings make four (4) groups: female offsprings (GIII and GIV), and male offspring (GV and GVI) pups of the control group and of the treated group, respectively. After the period of breastfeeding the animals (offsprings) were sacrificed after deep anesthesia and withdrew from the right knee in order to analyze the cartilaginous and bone tissue. The knee was fixed in 10% formaldehyde, decalcification and then subjected to histological processing for inclusion in paraffin. Slides were stained by H&E for morphometric analysis. The hormonal levels were assessed by ELISA and RIA. The results were subjected to statistical analysis by student-t. Results: depth to epiphyseal plate of the female offspring: resting zone (GI: 32,37 r 3,44 µm e GII: 37,32 r 3,42 µm, p < 0.05), proliferating zone (GI: 188,32 r 32,59 µm e GII: 213,18 r 23,06 µm, p < 0.05), e hypertrophic zone (GI: 372,59 r 45,50 µm e GII: 379,59 r 50,13 µm), e male offspring: resting zone (GIII: 33,18 r 3,55 µm e GIV: 34,88 r 3,15 µm), proliferating zone (GIII: 216,67 r 26,27 µm e GIV: 214,47 r 29,84 µm) e hypertrophic zone (GIII: 326,30 r 37,91 µm e GIV: 368,20 r 41,68 µm, p < 0.05). Conclusion: the morphometric results showed alteration of the epiphyseal disk thickness of the female and lactating mother pups with metoclopramide-induced hyperprolactinemia, a fact that suggests compromise in the development of bone growth.