Efeito do precondicionamento isquêmico remoto no transplante autólogo de ovário fresco e criopreservado em ratas

• Ano de defesa: 2009
• Autor(a) principal: Damous, Luciana Lamarão [UNIFESP]
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo (UNIFESP)
• Palavras-chave em Português: Ovário; Ratos; Transplante autólogo; Cirurgia experimental; Transplante de órgãos
• Link de acesso: http://repositorio.unifesp.br/handle/11600/9375

Objective: To evaluate remote ischemic preconditioning effect (PCI-R) in autologous fresh ovarian transplant and cryopreserved transplant in female rats. Methods: 97 young female Wistar EPM-1 rats were used in the experiment. They were divided in three study groups: Group 1: To determine the best PCI-R timing; Group 2: To evaluate the precocious and late repercussion of PCI-R in ovarian transplants (after 4, 7, 14, and 21 days); Group 3: To compare PCI-R effect in fresh and cryopreserved ovarian transplant, after 30 days. Vaginal smears, estradiol serum dosage, morphology, morphometry, neoangiogenesis by VEGF, proliferative activity by-ki-67 and apoptosis by caspasis-3 were evaluated. PCI-R was performed in the common iliac artery. Autologous ovarian tissue was implanted integrally without vascular anastomosis in the retro peritoneum, fixed with a non- absorbable stitch. Euthanasia was performed with a lethal dose of anesthetics used after material collection for analysis. Results: The best established PCI-R timing was 15 minutes when there was preservation of a greater number of viable ovarian follicles, high rate of cellular proliferation and neoangiogenesis. The animals that received transplants presented a larger number of viable ovarian follicles and corpora lutea than the ooforectomized groups. PCI-R interfered with follicular preservation at long term, inducing greater resistance in the late evaluation periods. After 30 days of the transplant, PCI-R caused an increase in the total ovarian follicles (mature and immature) and in the functioning of corpora lutea, with a tendency to increase vascularity, proliferative activity and less apoptotic activity in the late periods of evaluation. All transplanted groups presented higher hormonal levels than the control group. PCI-R caused precocious ovarian activity and increased the number of cycles in fresh transplants while in the cryopreserved transplants there was no interference. Conclusions: The 15 minute PCI-R presented a benefic effect on the transplanted ovarian tissue, inclusive in functional recovery, with a greater effect in the late post-transplant phases. The 14th day was a landmark in graft recovery and, from then on, the process of recovery was stabilized. PCI-R presented a better effect on the fresh transplanted tissue while in the cryopreserved transplant the evolution was good, independently of PCI-R.