Efeitos do extrato de cupuaçu sobre o estresse oxidativo e nitrosativo, marcadores inflamatórios e epigenéticos renais em modelo de diabetes mellitus

• Ano de defesa: 2017
• Autor(a) principal: Punaro, Giovana Rita [UNIFESP]
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo (UNIFESP)
• Palavras-chave em Português: Theobroma; Nitrosative stress; Inflammation; Epigenetic and kidney; Flavonoids; Desenvolvimento de compósitos; Efluente têxtil; Nanopartículas de magnetita; Remoção de óleos; Tratamento de águas; Flavonoides
• Link de acesso: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=5751123; http://repositorio.unifesp.br/handle/11600/50618

Objective: To evaluate the effects of the cupuaçu extract on nitrosative stress, inflammatory and epigenetic renal markers in diabetes mellitus model. Methods: Immortalized mouse mesangial cells (MiMC) were placed in medium with normal glucose (NG) or high glucose (HG), with or without extract of cupuaçu (EC, 500, 100, 50 or 10 μg/mL) during 24, 48 or 72 h for analysis of viability, proliferation, nitric oxide (NO) and reactive oxygen species (EROS). Male, adult Wistar rats were randomized into 4 groups: control (CTL) and diabetic (DM) who received water; CTLEC and DMEC who received 1 mL/day of EC (1 g/mL), via gavage for 8 weeks. The DM and DMEC rats became diabetic after receiving an intravenous injection of streptozotocin (45 mg/kg). After 8 weeks of treatment, metabolic profile and renal function were evaluated. The kidneys were collected and used for the analysis of NO, EROS and protein content of nitrotyrosine, eNOS, IL-6, IL-10, TNF-α, NF-kB p65, TGF-β1/2/3, iNOS, HDAC1, HDAC2 and HDAC3. Results: MiMC showed a normal viability in all groups, demonstrating that EC had no cytotoxic effects at studied doses and times. There was a significant increase in proliferation in the HG group, as well as in the NO and EROS levels, and these parameters were significantly reduced after EC treatment in the MiMC after 72 h. Treatment with EC in diabetic animals resulted in a partial normalization of metabolic and renal function parameters with significant reduction on NO and EROS production, nitrotyrosine, eNOS, IL-6, TGF-β1/2/3 and iNOS; as well as an increased HDAC3 compared to the DM group. Conclusion: Daily EC intake could contribute to better control of oxidative and nitrosative stress, associated with reduction of inflammatory factors and modulation of epigenetic, suggesting the importance of bioactive compounds of EC as non-pharmacological adjuvant to delay the early renal complications in diabetic patients.