Análise da via AKT/mTOR em ratos com doença renal crônica induzida por nefrectomia 5/6 submetidos a treinamento resistido prévio

• Ano de defesa: 2018
• Autor(a) principal: Saud, Alexandre [UNIFESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo (UNIFESP)
• Palavras-chave em Português: Chronic kidney disease; Exercise; Prevention & control; Doença renal crônica; Exercício; Prevenção & controle
• Link de acesso: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=6051357; https://repositorio.unifesp.br/handle/11600/52553

One of the main implications of chronic kidney disease (CKD) leads to a state of muscle atrophy. Resistance training is traditionally applied to increase muscle mass, however, there is a lack of complementary studies to clarify the mechanism of modulation that this type of training exercises on the renal system. We employed Wistar rats and submitted them to a resistance training, with the intention of reproduce the training of humans, under the following prescription: 6 to 12 climbs / day, 5 days / week, 10 weeks, counting on the adaptation, under training intensity 40 to 60% of the maximum load test. We divided the animals in five groups, SHAM and SNS (untrained), one group trained before nephrectomy (ENS), one trained group after nephrectomy (SNE) and one group trained before and after nephrectomy (ENE), all with 8 to 10 rats/group. In all groups were analyzed: renal weight, muscle weight, proteinuria, pro and anti-inflammatory cytokine levels, protein levels (Multiplex® assay). We observed that resistance exercise had a fundamental impact on the survival rate, especially in the ENE group compared to the sedentary with CKD. Under this exercise protocol, we observed that the EDL (type IIB) and plantar (type II) muscles presented greater weight in the trained groups, suggesting that the training avoided muscular catabolism. In addition, we found attenuated levels of creatinine clearance and proteinuria in the trained groups in relation to the sedentary group (p<0.05). Results suggested that the resistance training, especially the previous to the establishment of the disease, used in this study, acts as a modulator of the renal and muscular systems, and can be used so that the disease developed in low progression to a CKD, acting as nonpharmacological strategy, which is an adjunct to traditional treatment, in order to minimize the serious consequences of this disease.