Efeitos de diferentes intensidades de exercício resistido em biomarcadores de estresse oxidativo e na atividade das ectonucleotidases em plaquetas de ratos hipertensos

• Ano de defesa: 2016
• Autor(a) principal: Mello, Fábio Fernandes
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Santa Maria; Brasil; Bioquímica; UFSM; Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica; Centro de Ciências Naturais e Exatas
• Palavras-chave em Português: Hipertensão; Estresse oxidativo; Ectoenzimas; Exercício físico; Hypertension; Oxidative stress; Ectoenzymes; Physical exercise; CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
• Link de acesso: http://repositorio.ufsm.br/handle/1/17657

The objective of this study was to compare and analyze the effects of two 4-week resistance exercise protocols on oxidative stress (OS) biomarkers and ectonucleotidases activities in hypertensive rats. Male Wistar rats were divided into six groups (n = 10): control group, high-intensity exercise group (HIE), low intensity exercise group (LEL), the control group L-NAME, high-intensity exercise group L-NAME (HIE + L -name) and low intensity exercise group L-NAME (LIE + L-NAME). The results showed a significant reduction nitrite and nitrate oxidized content (NOx) in the L-NAME control group compared to the control group. There was a significant increase in NOx in the L-NAME exercise groups compared to their control group. A significant increase LIE + L-NAME in relation to HIE + L-NAME was verified. There was a significant reduction in protein carbonyl content in HIE and HIE + L-NAME group when compared to their control group. A significant increase in the levels of malondialdehyde (MDA) was observed in the L-NAME control group compared to the control group. In both L-NAME groups exercise significant reductions were observed in the MDA when compared to their control group. Significant reductions in L-NAME control group and L-NAME exercise groups were observed in the MDA compared with the control group. There was an increase in ATP hydrolysis in LIE when compared to the control group. Significant increases were observed in the hydrolysis of ADP and AMP in the L-NAME control group compared to the control group, and significant reductions in ADP and AMP in the L-NAME exercise groups compared to their control group. Significant increases in activity of adenosine deaminase (ADA) in HIE and LIE in the control group were observed. A significant reduction in ADA was observed in L-NAME control group compared to the control group. There was a significant increase in ADA in LIE + L-NAME compared to their respective controls. The results suggest that low intensity exercise may reduce the EO and regulate the activity of ectonucleotidases, reducing the deleterious effects of hypertension on the cardiovascular system, preventing cardiovascular complications of this disease.