Germinação in vitro de sementes e morfogênese de porongo (Lagenaria siceraria (Mol.) Standl.) e mogango(Cucurbita pepo L.)

Detalhes bibliográficos
Ano de defesa: 2005
Autor(a) principal: Silva, André Luís Lopes da
Orientador(a): Não Informado pela instituição
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de Santa Maria
BR
Agronomia
UFSM
Programa de Pós-Graduação em Agronomia
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Link de acesso: http://repositorio.ufsm.br/handle/1/5152
Resumo: The establishment and germination in vitro can supply a high amount of explants to develop morphogenesis protocols necessary for flowering induction in vitro, haploid and double-haploid plant production, clonal propagation, somatic embryogenesis among other applications. The objective was to develop protocols for the establishment and germination in vitro of bottle gourd (Lagenaria siceraria (Mol.) Standl.) and squash (Cucurbita pepo L.) species to effort morphogenesis studies. Seed disinfection treatments were done with ethanol 70% and NaOCl. Seed germination in vitro was evaluated changing medium-osmotic pressure, light availability, tegument and auxin presence, imbibition s time and scarification methods. Cotyledonary explants were used to induce direct organogenesis. Apical and nodal segments were grown in MS medium without growth regulators. Bottle gourd seeds did not germinate in culture medium, but it occurred on four layers of germitest paper and distilled water in the proportion of 1:7.5 (w/w). Light is not necessary for bottle gourd seed germination. Osmotic pressure reduction did not increase bottle gourd and squash germination. Bottle gourd apical and nodal segments regenerate in culture medium without growth regulators. Cotyledonary explants of bottle gourd and squash induce aerial growth, but in a low proliferation rate