Avaliação morfométrica e molecular de abelhas mandaçaias (Melipona spp.) da região da foz do rio São Francisco

• Ano de defesa: 2012
• Autor(a) principal: Calasans, Higor César Menezes
• Orientador(a): Ribeiro, Genésio Tâmara
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Sergipe
• Programa de Pós-Graduação: Pós-Graduação em Ecologia e Conservação
• País: BR
• Palavras-chave em Português: Melipona quadrifasciata; Morfometria; Distribuição espacial; PCR/RFLP
• Palavras-chave em Inglês: Melipona quadrifasciata; Geometric morphometry; Spatial distribution; PCR / RFLP
• Área do conhecimento CNPq: CNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL::CONSERVACAO DA NATUREZA
• Link de acesso: https://ri.ufs.br/handle/riufs/4436

In the genus Melipona, a bee Melipona quadrifasciata is one of the best known species and is divided into two subspecies M. q. quadrifasciata and M. q. anthidioides. This study aimed to evaluate the identity of bees mandaçaia the northern coast of Sergipe through morphometric and molecular techniques, and verify the possible geographic barrier represented by the Rio São Francisco. The survey was conducted in a total area of 30ha subdivided into three fragments in the municipalities of Piaçabuçu - AL, Brejo Grande SE, Ilha da Criminosa - SE. Were recorded in 14 colonies mandaçaia Brejo Grande and collected 10 specimens of each colony. No nest was located on the Ilha da Criminosa and Piaçabuçu, which reinforces the hypothesis that the Rio São Francisco could're representing a geographical barrier to the spread of the colonies. The spatial distribution of nests on a scale of 10ha, can be considered as random. Morphometry were used in the wings of the specimens collected and identified three other specimens of M. q. quadrifasciata, M. q. anthidioides and M. mandacaia. The data were subjected to cluster analyzes, principal component analysis, canonical variate analysis, Procrustes analysis of variance test and correlation matrices. Analyses of morphometric data are congruent and identified the M. q. quadrifascita as the identity of the species collected. The analysis of cross-validation and correlation matrices showed no significance, showing great similarity intrapopulation. Total DNA from each sample was subjected to analysis of PCR / RFLP (enzymes: Taq I, Vsp I and Mbo II), identifying a unique pattern of haplotypes for the bee population of the mouth, showing the high genetic similarity. All nests were located in palm trees that would be condemned by the low production due to damage caused by pests, besides the very low supply of other sites for nesting, which sets very high risk of population extinction.